概述
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产品名称人FGF9 ELISA试剂盒
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检测方法Colorimetric
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样品类型Cell culture supernatant, Plasma
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检测类型Sandwich (quantitative)
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灵敏度< 200 pg/ml
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范围358.4 pg/ml - 35000 pg/ml
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回收率
97 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 100.27 88% - 107% Plasma 94.38 83% - 102% -
实验步骤Multiple steps standard assay
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种属反应性与反应: Human
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产品概述
Abcam’s FGF9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF9 in plasma and cell culture supernatants. (Human FGF9 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human FGF9 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human FGF9 in normal serum with ab100520, only in serum spiked with Human FGF9.
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说明
Optimization may be required with urine samples.
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经测试应用适用于: Sandwich ELISAmore details
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平台Microplate
性能
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存放说明Store at -20°C. Please refer to protocols.
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组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human FGF9 2 vials FGF9 Microplate (12 strips x 8 wells) 1 unit Recombinant Human FGF9 Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
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组织特异性Glial cells.
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疾病相关Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3) [MIM:612961]. Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness.
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序列相似性Belongs to the heparin-binding growth factors family.
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翻译后修饰Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.
N-glycosylated. -
细胞定位Secreted.
- Information by UniProt
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别名
- Elbow knee synostosis
- FGF 9
- FGF-9
see all -
数据库链接
- Entrez Gene: 2254 Human
- Omim: 600921 Human
- SwissProt: P31371 Human
- Unigene: 111 Human
应用
Our Abpromise guarantee covers the use of ab100520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
图片
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Typical Standard CurveRepresentative Standard Curve using ab100520.
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Typical Standard CurveRepresentative Standard Curve using ab100520.
实验方案
文献
ab100520 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"