概述

  • 产品名称人FGF7 ELISA试剂盒
    参阅全部 FGF7 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.5 pg/ml
  • 范围
    0.55 pg/ml - 400 pg/ml
  • 复检

    > 93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 93.24 81% - 98%
    Serum 112.9 102% - 123%
    Plasma 126.2 114% - 135%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s FGF7 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF7 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human FGF7 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Fibroblast growth factors (FGFs) can stimulate the proliferation of mesenchymal, epithelial and neuroectodermal cells. Members of the family include Acidic FGF (also known as aFGF or FGF 1), basic FGF (bFGF or FGF 2), FGF3 (also known as Int2) and FGF4 (also known as hst or Kaposi FGF). FGF4 was first revealed to be an oncoprotein in Kaposi's sarcoma cells. Other family members include FGF5, FGF6, FGF7 (keratinocyte growth factor, KGF), FGF8 (AIGF), FGF9 (GAF) and FGF10.
  • 细胞定位Secreted
  • 别名
    • FGF 7
    • Fibroblast growth factor 7
    • HBGF 7
    • Heparin binding growth factor 7
    • Keratinocyte growth factor
    • KGF
    • OTTMUSP00000016341
    • OTTMUSP00000016342
    • RP23-386I21.3
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human FGF7 ELISA Kit 图像

  • Representative Standard Curve using ab100519

实验方案

Human FGF7 ELISA Kit (ab100519)参考文献

ab100519 has not yet been referenced specifically in any publications.

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