概述

  • 产品名称
    人FGF6 ELISA试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 6 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.42 84% - 103%
    Plasma 94.63 82% - 102%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s FGF6 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF6 in plasma and cell culture supernatants. Collect plasma using EDTA as an anticoagulant. Heparin and citrate are not recommended. (Human FGF6 concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human FGF6 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF6 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm.


    We have not been able to detect the endogenous Human FGF6 in normal serum with ab100518, only in serum spiked with Human FGF6.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human FGF6 2 vials
    FGF6 Microplate (12 strips x 8 wells) 1 unit
    Recombinant Human FGF6 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 相关性
    FGF6 is a secreted heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF6 is expressed in leukemia cell lines with platelet megakaryocytic differentiation potential. It signals through FGFR 1c, 2c, and 4.
  • 别名
    • FGF 6
    • Fibroblast growth factor 6
    • Fibroblast growth factor 6 precursor
    • HBGF 6
    • HBGF6
    • Heparin binding growth factor 6
    • Heparin secretory transforming protein 2
    • HST 2
    • HST2
    • HSTF 2
    • HSTF2
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab100518.

  • Representative Standard Curve using ab100518.

实验方案

文献

ab100518 has not yet been referenced specifically in any publications.

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