概述

  • 产品名称人FGF4 ELISA试剂盒
    参阅全部 FGF4 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 50 pg/ml
  • 范围
    24.69 pg/ml - 18000 pg/ml
  • 复检

    92 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture extracts 93.45 94% - 103%
    Tissue Extracts 91.96 82% - 102%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s FGF4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF4 in cell lysates and tissue lysates.

    This assay employs an antibody specific for Human FGF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性FGF4 is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF4 signals through the FGFR 1c, 2c, 3c, and 4.
  • 别名
    • Fibroblast growth factor 4
    • HBGF4
    • Heparin binding growth factor 4
    • Heparin secretory transforming protein 1
    • HST
    • HST1
    • HSTF1
    • KS3
    • Transforming protein KS3
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human FGF4 ELISA Kit 图像

  • Representative standard curve using ab100517

实验方案

Human FGF4 ELISA Kit (ab100517)参考文献

ab100517 has not yet been referenced specifically in any publications.

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