概述

  • 产品名称
    人FGF4 ELISA试剂盒
    参阅全部 FGF4 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 50 pg/ml
  • 范围
    24.69 pg/ml - 18000 pg/ml
  • 复检

    > 90 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 91.54 80% - 103%
    Plasma 96.21 88% - 110%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s FGF4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF4 in plasma and cell culture supernatants.  (Human FGF4 concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human FGF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Human FGF4 in normal serum with ab100516, only in serum spiked with Human FGF4.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab100516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab100516

  • Representative Standard Curve using ab100516

实验方案

文献

ab100516 has not yet been referenced specifically in any publications.

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