概述

  • 产品名称人FGF basic ELISA试剂盒(FGF2)
    参阅全部 FGF basic 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 50 pg/ml
  • 范围
    102.4 pg/ml - 10000 pg/ml
  • 复检

    > 95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture extracts 97.65 87% - 108%
    Tissue Extracts 95.87 86% - 107%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s FGF basic (FGF2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF basic in cell lysates and tissue lysates.

    This assay employs an antibody specific for Human FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
  • 别名
    • Basic fibroblast growth factor
    • BFGF
    • FGF 2
    • FGF B
    • FGF2
    • FGF2 basic
    • FGFB
    • Fibroblast growth factor 2 (basic)
    • HBGF 2
    • HBGF2
    • HBGH 2
    • Heparin binding growth factor 2
    • Heparin binding growth factor 2 precursor
    • OTTMUSP00000046776
    • Prostatropin
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab99980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human FGF basic ELISA Kit (FGF2) 图像

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • FGF2 measured in tissue lysates showing quantity (pg) per 1 mg of extracted protein. Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were diluted 200-2000 fold.

  • FGF2 measured in cell culture lysates showing quantity (pg) per 1 mln cells. Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 200-2000 fold.

实验方案

Human FGF basic ELISA Kit (FGF2) (ab99980)参考文献

This product has been referenced in:
  • Jaszberenyi M  et al. Potentiation of cytotoxic chemotherapy by growth hormone-releasing hormone agonists. Proc Natl Acad Sci U S A 111:781-6 (2014). Read more (PubMed: 24379381) »

See 1 Publication for this product

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Unfortunately, we have not tested the cross-reactivity for ab99980 with mouse sample. Thus, we do not have cross reactivity data for mouse sample with ab99980. Epitope mapping has also not been performed. At the m...

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Both the primary antibody specific for human FGF and the biotinylated secondary antibody used in this kit are monoclonal.

Unfortunately we do not sell these antibodies separately however, we do sell anti...

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Unfortunately, epitope mapping of the two antibody pairs has not been performed, so we do not know the exact amino acid sequence recognised and if these regions are specific for human and mouse, respect...

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