人FGF basic ELISA试剂盒(FGF2) (ab99979)

概述

  • 产品名称人FGF basic ELISA试剂盒(FGF2)
    参阅全部 FGF basic 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Cell Lysate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 50 pg/ml
  • 范围
    102.4 pg/ml - 10000 pg/ml
  • 复检

    94 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 93.48 83% - 103%
    Serum 95.46 84% - 104%
    Plasma 92.64 82% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
    预测可用于: Pig
  • 产品概述

    Abcam’s FGF basic (FGF2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF basic in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
  • 别名
    • Basic fibroblast growth factor
    • BFGF
    • FGF 2
    • FGF B
    • FGF2
    • FGF2 basic
    • FGFB
    • Fibroblast growth factor 2 (basic)
    • HBGF 2
    • HBGF2
    • HBGH 2
    • Heparin binding growth factor 2
    • Heparin binding growth factor 2 precursor
    • OTTMUSP00000046776
    • Prostatropin
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab99979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human FGF basic ELISA Kit (FGF2) 图像

  • Representative Standard Curve using ab99979.

  • Representative Standard Curve using ab99979.

实验方案

Human FGF basic ELISA Kit (FGF2) (ab99979)参考文献

ab99979 has not yet been referenced specifically in any publications.

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Vielen Dank für Ihren Anruf.

Leider hat das Labornicht einpräzipitiertes FGF getestet in diesem ELISA, weder ein FGF präzipitiert mit Ammonium Sulfat oder Säure. Das Labor denkt auch, dass 10kD cut off (oder 5kD cut off) s...

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Thank you for contacting us with your enquiry, and for your patience while I have been in touch with the lab for more information.

We have not tested any of these kits in-house with cerebrospinal fluid, but the lab scientistsrecommend using a...

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These kits are provided with 12 strips of 8 wells each in a frame that is the same size as a standard 96-well plate (˜5 x 3.33"). If you need any more specific information, please let me know and I will be happy t...

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Thank you for contacting us.

This kit is suitable for FGF basic detection in serum, plasma, cell culture medium and urine.

The kit is sensitive to detect the FGFbasic as lower than 50pg/ml. If customer is interested in testing the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"