概述

  • 产品名称人FGF-21 ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.7%
    批次间
    样品 n Mean SD CV%
    Overall 7.2%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.03 ng/ml
  • 范围
    0.031 ng/ml - 2 ng/ml
  • 复检

    97 %

  • 检测时间
    5h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s FGF-21 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human FGF-21 in plasma, serum, and cell culture supernatants.

    A FGF-21 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a FGF-21 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of FGF-21 captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.
  • 序列相似性Belongs to the heparin-binding growth factors family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • FGF 21
    • FGF-21
    • Fgf21
    • FGF21_HUMAN
    • Fibroblast growth factor 21
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab125966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human FGF-21 ELISA Kit 图像

  • FGF-21 measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab125966

实验方案

Human FGF-21 ELISA Kit (ab125966)参考文献

ab125966 has not yet been referenced specifically in any publications.

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