人Fetuin A ELISA试剂盒(AHSG) (ab119594)

概述

  • 产品名称人Fetuin A ELISA试剂盒(AHSG)
    参阅全部 Fetuin A 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    312 pg/ml - 20000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human Fetuin A (AHSG) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Fetuin A (AHSG) in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Fetuin A (AHSG) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Fetuin A (AHSG) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Fetuin A (AHSG) amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
  • 组织特异性Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
  • 序列相似性Belongs to the fetuin family.
    Contains 2 cystatin fetuin-A-type domains.
  • 翻译后修饰Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • A2HS
    • AHS
    • Ahsg
    • Alpha 2 HS glycoprotein
    • Alpha-2-HS-glycoprotein chain B
    • Alpha-2-Z-globulin
    • Ba-alpha-2-glycoprotein
    • FETUA
    • FETUA_HUMAN
    • Fetuin-A
    • HSGA
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Fetuin A ELISA Kit (AHSG) 图像

  • Representative Standard Curve using ab119594

实验方案

Human Fetuin A ELISA Kit (AHSG) (ab119594)参考文献

ab119594 has not yet been referenced specifically in any publications.

Product Wall

ab119594 has not been tested with mouse samples, and we do not recommend it for use with mouse samples. We also do not recommend it's use with rat samples either.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"