概述

  • 产品名称人Ferritin (FTL) ELISA试剂盒
    参阅全部 Ferritin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.2%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 1.5 ng/ml
  • 范围
    1.563 ng/ml - 50 ng/ml
  • 复检

    97 %

  • 检测时间
    4h 0m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
    预测可用于: Monkey
  • 产品概述

    Abcam’s Ferritin (FTL) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ferritin concentrations in plasma, serum, milk, cerebrospinal fluid and cell culture supernatants.


    A Ferritin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ferritin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ferritin captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Ferritin ELISA Kit (FLT) (ab200018) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • 疾病相关Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
  • 序列相似性Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt
  • 别名
    • ferritin L chain
    • Ferritin L subunit
    • Ferritin light chain
    • ferritin light polypeptide
    • ferritin light polypeptide like 3
    • FRIL_HUMAN
    • FTL
    • NBIA3
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab108837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Ferritin (FTL) ELISA Kit 图像

  • Human Ferritin measured in biological fluids showing quantity (pg) per mL of tested sample. Serum and plasma samples were diluted 10-20 fold. Milk, urine and PBMC supernatant were diluted 1-4 fold.

  • Human Ferritin standard curve: mean of duplicates (+/- SD)
  • Human Ferritin measured in biological fluids showing quantity (pg) per mL of tested sample.
  • Representative Standard Curve using ab108837

实验方案

Human Ferritin (FTL) ELISA Kit (ab108837)参考文献

This product has been referenced in:
  • Gomes JA  et al. Brain iron metabolism and brain injury following subarachnoid hemorrhage: iCeFISH-pilot (CSF iron in SAH). Neurocrit Care 21:285-93 (2014). ELISA ; Human . Read more (PubMed: 24710655) »
  • Yu SY  et al. Sleep disorders in Parkinson's disease: clinical features, iron metabolism and related mechanism. PLoS One 8:e82924 (2013). Sandwich ELISA ; Human . Read more (PubMed: 24376607) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.


The proper value in human plasma is 8-400 ng/ml. On average we get 60 ng/ml for our samples.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more ad...

Read More



I can confirm that this kit is suitable for EDTA collected plasma.

ab108698 Ferritin Human ELISA Kit: for pre-treatment of serum samples we recommend HEPES buffer.

ab108837 Ferritin Human ELISA KIt: I am sorry this is not tested with other buffers, so we would not be able to advise which buffer to use. Ther...

Read More

One immunogen is a recombinant protein and the other one is a purified protein.

Thank you very much for your reply. My colleague Jackie is out of the office today but I'll be happy to help you with this.

Do you need antibodies that are specific for ferritin heavy or ferritin light chains? Looking through our catalog, I o...

Read More

Thank you for contacting us. We have a kit that may be suitable for your requirements. Ferritin Human ELISA KIt (ab108837) is designed for detection of Human Ferritin in plasma, serum, milk, and cell culture supernatants. This assay employs a quant...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"