概述

  • 产品名称
    人Ferritin (FTL) ELISA试剂盒
    参阅全部 Ferritin 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.2%
  • 样品类型
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 1.5 ng/ml
  • 范围
    1.563 ng/ml - 50 ng/ml
  • 复检

    97 %

  • 检测时间
    4h 0m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
    预测可用于: Monkey
  • 产品概述

    Abcam’s Ferritin (FTL) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ferritin concentrations in plasma, serum, milk, cerebrospinal fluid and cell culture supernatants.


    A Ferritin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ferritin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ferritin captured in plate.


    Get results in 90 minutes with Human Ferritin ELISA Kit (FLT) (ab200018) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 20ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Ferritin Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Ferritin Microplate (12 x 8 well strips) 1 unit
    Ferritin Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究领域
  • 功能
    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • 疾病相关
    Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
  • 序列相似性
    Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt
  • 别名
    • ferritin L chain
    • Ferritin L subunit
    • Ferritin light chain
    • ferritin light polypeptide
    • ferritin light polypeptide like 3
    • FRIL_HUMAN
    • FTL
    • NBIA3
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab108837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

图片

  • Human Ferritin measured in biological fluids showing quantity (pg) per mL of tested sample. Serum and plasma samples were diluted 10-20 fold. Milk, urine and PBMC supernatant were diluted 1-4 fold.

  • Human Ferritin standard curve: mean of duplicates (+/- SD)
  • Human Ferritin measured in biological fluids showing quantity (pg) per mL of tested sample.
  • Representative Standard Curve using ab108837

实验方案

文献

This product has been referenced in:
  • Hu Y  et al. Investigation on Abnormal Iron Metabolism and Related Inflammation in Parkinson Disease Patients with Probable RBD. PLoS One 10:e0138997 (2015). ELISA ; Human . Read more (PubMed: 26431210) »
  • Gomes JA  et al. Brain iron metabolism and brain injury following subarachnoid hemorrhage: iCeFISH-pilot (CSF iron in SAH). Neurocrit Care 21:285-93 (2014). ELISA ; Human . Read more (PubMed: 24710655) »

See all 3 Publications for this product

客户评价及客户问答

Thank you for contacting us.


The proper value in human plasma is 8-400 ng/ml. On average we get 60 ng/ml for our samples.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more ad...

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I can confirm that this kit is suitable for EDTA collected plasma.

ab108698 Ferritin Human ELISA Kit: for pre-treatment of serum samples we recommend HEPES buffer.

ab108837 Ferritin Human ELISA KIt: I am sorry this is not tested with other buffers, so we would not be able to advise which buffer to use. Ther...

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One immunogen is a recombinant protein and the other one is a purified protein.

Thank you very much for your reply. My colleague Jackie is out of the office today but I'll be happy to help you with this.

Do you need antibodies that are specific for ferritin heavy or ferritin light chains? Looking through our catalog, I o...

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Thank you for contacting us. We have a kit that may be suitable for your requirements. Ferritin Human ELISA KIt (ab108837) is designed for detection of Human Ferritin in plasma, serum, milk, and cell culture supernatants. This assay employs a quant...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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