人Ferritin ELISA试剂盒(ab108698)

概述

  • 产品名称人Ferritin ELISA试剂盒
    参阅全部 Ferritin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Control 48 < 5.4%
    批次间
    样品 n Mean SD CV%
    Control 48 < 6.1%
  • 测试
    1 x 96 test
  • 样品类型
    Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.53 ng/ml
  • 复检

    98.66 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Ferritin competitive Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Ferritin in serum and plasma.


    A 96-well plate has been precoated with anti-Ferritin IgG antibodies. Samples, standards and the Ferritin-HRP conjugate are added to the wells, where Ferritin in the sample and standards binds to the precoated antibody and added Ferritin-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Ferritin in the sample and the intensity is measured at 450 nm.


    Save time with a 90-minute ELISA protocol. Try Human Ferritin ELISA Kit (FLT) (ab200018) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 存放说明Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests
    10X Washing Solution 1 x 50ml
    Anti-Ferritin IgG Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit
    Anti-Ferritin-HRP conjugate 1 x 12ml
    Cover foil 1 unit
    Ferritin Control 1 x 1ml
    Ferritin Standard 0 - 0 ng/mL 1 x 3ml
    Ferritin Standard 1 - 5 ng/mL 1 x 1ml
    Ferritin Standard 2 - 20 ng/mL 1 x 1ml
    Ferritin Standard 3 - 100 ng/mL 1 x 1ml
    Ferritin Standard 4 - 400 ng/mL 1 x 1ml
    Ferritin Standard 5 - 1,000 ng/mL 1 x 1ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • 研究领域
  • 功能Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • 疾病相关Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
  • 序列相似性Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt
  • 别名
    • ferritin L chain
    • Ferritin L subunit
    • Ferritin light chain
    • ferritin light polypeptide
    • ferritin light polypeptide like 3
    • FRIL_HUMAN
    • FTL
    • NBIA3
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Ferritin ELISA Kit 图像

  • Representative Standard Curve using ab108698

实验方案

Human Ferritin ELISA Kit (ab108698)参考文献

ab108698 has not yet been referenced specifically in any publications.

Product Wall

ab108698 Ferritin Human ELISA Kit: for pre-treatment of serum samples we recommend HEPES buffer.

ab108837 Ferritin Human ELISA KIt: I am sorry this is not tested with other buffers, so we would not be able to advise which buffer to use. Ther...

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The c-Kit Human ELISA Kit (ab45924) and Ferritin Human ELISA Kit (ab108698) both come with strip wells provided.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"