人Ferritin ELISA试剂盒(ab108698)
Key features and details
- Sensitivity: 0.53 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Ferritin ELISA试剂盒
参阅全部 Ferritin Light Chain 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Control 48 < 5.4% 批次间 样品 n Mean SD CV% Control 48 < 6.1% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
0.53 ng/ml -
回收率
98.66 %
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实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human Ferritin ELISA kit is a competitive ELISA designed for the accurate quantitative measurement of Ferritin (FTL) in serum and plasma.
A 96-well plate has been precoated with anti-Ferritin IgG antibodies. Samples, standards and the Ferritin-HRP conjugate are added to the wells, where Ferritin in the sample and standards binds to the precoated antibody and added Ferritin-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Ferritin in the sample and the intensity is measured at 450 nm.
Get results in 90 minutes with Human Ferritin ELISA Kit (FLT) (ab200018) from our SimpleStep ELISA® range.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 1 x 96 tests 10X Washing Solution 1 x 50ml 1 x 50ml Anti-Ferritin IgG Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit 1 unit Anti-Ferritin-HRP conjugate 1 x 12ml 1 x 12ml Cover foil 1 unit 1 unit Ferritin Control 1 x 1ml 1 x 1ml Ferritin Standard 0 - 0 ng/mL 1 x 3ml 1 x 3ml Ferritin Standard 1 - 5 ng/mL 1 x 1ml 1 x 1ml Ferritin Standard 2 - 20 ng/mL 1 x 1ml 1 x 1ml Ferritin Standard 3 - 100 ng/mL 1 x 1ml 1 x 1ml Ferritin Standard 4 - 400 ng/mL 1 x 1ml 1 x 1ml Ferritin Standard 5 - 800 ng/mL 1 x 1ml 1 x 1ml Stop Solution 1 x 15ml 1 x 15ml Strip holder 1 unit 1 unit TMB Substrate Solution 1 x 15ml 1 x 15ml -
研究领域
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功能
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. -
疾病相关
Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. -
序列相似性
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. - Information by UniProt
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别名
- Ferritin L chain
- Ferritin L subunit
- Ferritin light chain
see all -
数据库链接
- Entrez Gene: 2512 Human
- Omim: 134790 Human
- SwissProt: P02792 Human
- Unigene: 433670 Human
- Unigene: 713706 Human
相关产品
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Related Products
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab108698 被引用在 7 文献中.
- Raha AA et al. Hepcidin Increases Cytokines in Alzheimer's Disease and Down's Syndrome Dementia: Implication of Impaired Iron Homeostasis in Neuroinflammation. Front Aging Neurosci 13:653591 (2021). PubMed: 33994996
- Raha-Chowdhury R et al. Impaired Iron Homeostasis and Haematopoiesis Impacts Inflammation in the Ageing Process in Down Syndrome Dementia. J Clin Med 10:N/A (2021). PubMed: 34209847
- Ajepe AA et al. Prevalence and foetomaternal effects of iron deficiency anaemia among pregnant women in Lagos, Nigeria. PLoS One 15:e0227965 (2020). PubMed: 31971986
- Richardson KJ et al. Mechanical sensitivity of red blood cells improves in individuals with hemochromatosis following venesection therapy. Transfusion 60:3001-3009 (2020). PubMed: 32939772
- Richardson KJ et al. Hemochromatosis alters the sensitivity of red blood cells to mechanical stress. Transfusion 60:2982-2990 (2020). PubMed: 32945551
- Devos D et al. A ferroptosis-based panel of prognostic biomarkers for Amyotrophic Lateral Sclerosis. Sci Rep 9:2918 (2019). PubMed: 30814647
- Smith EM et al. High-dose vitamin D3 reduces circulating hepcidin concentrations: A pilot, randomized, double-blind, placebo-controlled trial in healthy adults. Clin Nutr 36:980-985 (2017). PubMed: 27402475