人Fc-gamma RIIB/C ELISA试剂盒(ab113333)

概述

  • 产品名称人Fc-gamma RIIB/C ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 85 pg/ml
  • 范围
    78.13 pg/ml - 5000 pg/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 88.89 79% - 99%
    Serum 100 91% - 108%
    Plasma 112.1 96% - 135%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Fc-gamma RIIB/C (Fc gamma Receptor II B/C) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Fc-gamma RIIB/C in serum, plasma, cell culture supernatants.

    This assay employs an antibody specific for Human Fc-gamma RIIB/C coated on a 96- well plate. Standards and samples are pipetted into the wells and Fc-gamma RIIB/C present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Fc-gamma RIIB/C antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fc-gamma RIIB/C bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

应用

Our Abpromise guarantee covers the use of ab113333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Fc-gamma RIIB/C ELISA Kit 图像

  • Representative Standard Curve using ab113333

  • Representative Standard Curve using ab113333

实验方案

Human Fc-gamma RIIB/C ELISA Kit (ab113333)参考文献

ab113333 has not yet been referenced specifically in any publications.

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