人Fatty Acid Oxidation In-Cell ELISA试剂盒(ab118182)

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概述

  • 产品名称
    人Fatty Acid Oxidation In-Cell ELISA试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型
    Cell-based (quantitative)
  • 实验步骤
    Multiple steps standard assay
  • 产品概述

    ab118182 uses quantitative immunocytochemistry to measure protein levels or post-translational modifications in cultured cells. Cells are fixed in a microplate and targets of interest are detected with highly specific, well-characterized monoclonal antibodies, and levels are quantified with IRDye®-labeled Secondary Antibodies. IR imaging and quantitation is performed using a LI-COR® Odyssey® or Aerius® system.

     

    Plates are available in our ICE (In-Cell ELISA) Support Pack (ab111542) which can be bought seperately.

  • 说明

    Upon receipt spin down the contents of the IRDye®-labeled Secondary Antibody tube and protect from light. Store all components upright at 4C. This kit is stable for at least 6 months from receipt.

  • 经测试应用
    适用于: In-Cell ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab118182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
In-Cell ELISA Use at an assay dependent concentration.

图片

  • Immunocytochemistry - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
    Immunocytochemistry - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
    Antibody specificity demonstrated by immunocytochemistry. Visualization under the microscope was carried out with a completely opened aperture and a very narrow field of visualization at 40x. Panel A shows control fibroblasts and panel B shows deficient fibroblasts. Left panel shows staining with anti-ACADVL ab, center panel with anti-ACADM ab and right panel with anti-HADHA ab. Note that although LCHAD#1 deficient had a characterized homozygous mutation, the antibody shows a mosaic pattern of staining with about 1/3 of the cells lacking HADHA staining and an overall 40% reduction of signal as observed by ICE.
  • In-Cell ELISA - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
    In-Cell ELISA - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
    Panel B shows levels of ACADM protein in two deficient cell lines (ACADM:p. [K304E]).
  • In-Cell ELISA - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
    In-Cell ELISA - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
    Panel C shows levels of HADHA protein in two deficient cell lines: (1) HADHA:p.[E474Q] and (2) HADHB:p. [R61H];[R247H]. Note that although LCHAD#1 deficient has a partial deficiency as observed by ICC.
  • In-Cell ELISA - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
    In-Cell ELISA - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
    Panel A shows levels of ACADVL protein in a deficient cell line (ACADVL:p.[N122D]).

实验方案

文献

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