人Fatty Acid Oxidation In-Cell ELISA试剂盒(ab118182)

概述

  • 产品名称人Fatty Acid Oxidation In-Cell ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Cell-based (quantitative)
  • 实验步骤Multiple steps standard assay
  • 产品概述

    ab118182 uses quantitative immunocytochemistry to measure protein levels or post-translational modifications in cultured cells. Cells are fixed in a microplate and targets of interest are detected with highly specific, well-characterized monoclonal antibodies, and levels are quantified with IRDye®-labeled Secondary Antibodies. IR imaging and quantitation is performed using a LI-COR® Odyssey® or Aerius® system.

     

    Plates are available in our ICE (In-Cell ELISA) Support Pack (ab111542) which can be bought seperately.

  • 说明

    Upon receipt spin down the contents of the IRDye®-labeled Secondary Antibody tube and protect from light. Store all components upright at 4C. This kit is stable for at least 6 months from receipt.

  • 经测试应用适用于: In-Cell ELISAmore details
  • 平台Microplate

性能

    应用

    Our Abpromise guarantee covers the use of ab118182 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    In-Cell ELISA Use at an assay dependent concentration.

    Human Fatty Acid Oxidation In-Cell ELISA Kit 图像

    • Antibody specificity demonstrated by immunocytochemistry. Visualization under the microscope was carried out with a completely opened aperture and a very narrow field of visualization at 40x. Panel A shows control fibroblasts and panel B shows deficient fibroblasts. Left panel shows staining with anti-ACADVL ab, center panel with anti-ACADM ab and right panel with anti-HADHA ab. Note that although LCHAD#1 deficient had a characterized homozygous mutation, the antibody shows a mosaic pattern of staining with about 1/3 of the cells lacking HADHA staining and an overall 40% reduction of signal as observed by ICE.
    • Panel B shows levels of ACADM protein in two deficient cell lines (ACADM:p. [K304E]).
    • Panel C shows levels of HADHA protein in two deficient cell lines: (1) HADHA:p.[E474Q] and (2) HADHB:p. [R61H];[R247H]. Note that although LCHAD#1 deficient has a partial deficiency as observed by ICC.
    • Panel A shows levels of ACADVL protein in a deficient cell line (ACADVL:p.[N122D]).

    实验方案

    Human Fatty Acid Oxidation In-Cell ELISA Kit (ab118182)参考文献

    ab118182 has not yet been referenced specifically in any publications.

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