ab118182 uses quantitative immunocytochemistry to measure protein levels or post-translational modifications in cultured cells. Cells are fixed in a microplate and targets of interest are detected with highly specific, well-characterized monoclonal antibodies, and levels are quantified with IRDye®-labeled Secondary Antibodies. IR imaging and quantitation is performed using a LI-COR® Odyssey® or Aerius® system.
Plates are available in our ICE (In-Cell ELISA) Support Pack (ab111542) which can be bought seperately.
Upon receipt spin down the contents of the IRDye®-labeled Secondary Antibody tube and protect from light. Store all components upright at 4C. This kit is stable for at least 6 months from receipt.
组件 | 1 x 96 tests |
---|---|
1000X IRDye 800-labeled Secondary antibody | 1 x 0.036ml |
100X ACADM Primary Antibody | 1 x 0.12ml |
100X ACADVL Primary Antibody | 1 x 0.12ml |
100X HADHA Primary Antibody | 1 x 0.12ml |
10X Blocking Buffer | 1 x 20ml |
10X Phosphate Buffered Saline | 1 x 100ml |
33x Triton X-100 | 1 x 5ml |
400X Tween-20 | 1 x 4ml |
Janus Green Stain | 1 x 17ml |
Our Abpromise guarantee covers the use of ab118182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
In-Cell ELISA | Use at an assay dependent concentration. |
ab118182 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"