人Fatty Acid Oxidation In-Cell ELISA试剂盒(ab118182)

概述

• 产品名称
  人Fatty Acid Oxidation In-Cell ELISA试剂盒
• 检测方法
  Colorimetric
• 样品类型
  Adherent cells, Suspension cells
• 检测类型
  Cell-based (quantitative)
• 实验步骤
  Multiple steps standard assay
• 产品概述

  ab118182 uses quantitative immunocytochemistry to measure protein levels or post-translational modifications in cultured cells. Cells are fixed in a microplate and targets of interest are detected with highly specific, well-characterized monoclonal antibodies, and levels are quantified with IRDye®-labeled Secondary Antibodies. IR imaging and quantitation is performed using a LI-COR® Odyssey® or Aerius® system.

   

  Plates are available in our ICE (In-Cell ELISA) Support Pack (ab111542) which can be bought seperately.

• 说明

  Upon receipt spin down the contents of the IRDye®-labeled Secondary Antibody tube and protect from light. Store all components upright at 4C. This kit is stable for at least 6 months from receipt.

• 经测试应用
  适用于: In-Cell ELISAmore details
• 平台
  Microplate

性能

• 存放说明
  Store at +4°C. Please refer to protocols.

• 组件	1 x 96 tests
  1000X IRDye 800-labeled Secondary antibody	1 x 0.036ml
  100X ACADM Primary Antibody	1 x 0.12ml
  100X ACADVL Primary Antibody	1 x 0.12ml
  100X HADHA Primary Antibody	1 x 0.12ml
  10X Blocking Buffer	1 x 20ml
  10X Phosphate Buffered Saline	1 x 100ml
  33x Triton X-100	1 x 5ml
  400X Tween-20	1 x 2ml
  Janus Green Stain	1 x 17ml

• 研究领域
  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Fatty Acids
  • Metabolism

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Fatty acids

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Fatty acid oxidation

Human Fatty Acid Oxidation In-Cell ELISA Kit 图像

• 

  Immunocytochemistry - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
  Antibody specificity demonstrated by immunocytochemistry. Visualization under the microscope was carried out with a completely opened aperture and a very narrow field of visualization at 40x. Panel A shows control fibroblasts and panel B shows deficient fibroblasts. Left panel shows staining with anti-ACADVL ab, center panel with anti-ACADM ab and right panel with anti-HADHA ab. Note that although LCHAD#1 deficient had a characterized homozygous mutation, the antibody shows a mosaic pattern of staining with about 1/3 of the cells lacking HADHA staining and an overall 40% reduction of signal as observed by ICE.
• 

  In-Cell ELISA - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
  Panel B shows levels of ACADM protein in two deficient cell lines (ACADM:p. [K304E]).
• 

  In-Cell ELISA - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
  Panel C shows levels of HADHA protein in two deficient cell lines: (1) HADHA:p.[E474Q] and (2) HADHB:p. [R61H];[R247H]. Note that although LCHAD#1 deficient has a partial deficiency as observed by ICC.
• 

  In-Cell ELISA - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
  Panel A shows levels of ACADVL protein in a deficient cell line (ACADVL:p.[N122D]).