人Fas Ligand ELISA试剂盒(APTL) (ab100515)

概述

  • 产品名称人Fas Ligand ELISA试剂盒(APTL)
    参阅全部 Fas Ligand 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 2 pg/ml
  • 范围
    1.37 pg/ml - 1000 pg/ml
  • 复检

    75 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 76.99 70% - 90%
    Serum 76.36 70% - 87%
    Plasma 73.42 67% - 86%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Fas Ligand (APTL) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Fas Ligand in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Fas Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Fas Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Fas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system.
  • 细胞定位Cell Membrane (single-pass type II membrane protein) and Secreted
  • 别名
    • Apoptosis (APO 1) antigen ligand 1
    • Apoptosis antigen ligand
    • APT1LG1
    • APTL
    • CD178
    • CD95 L
    • CD95 ligand
    • CD95L
    • CD95L protein
    • Fas antigen ligand
    • FASL
    • FASLG
    • TNF superfamily member 6
    • TNFSF6
    • Tumor necrosis factor (ligand) superfamily member 6
    • Tumor necrosis factor ligand superfamily member 6
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Fas Ligand ELISA Kit (APTL) 图像

  • Representative Standard Curve using ab100515.

  • Representative Standard Curve using ab100515.

实验方案

Human Fas Ligand ELISA Kit (APTL) (ab100515)参考文献

ab100515 has not yet been referenced specifically in any publications.

Product Wall

good working Elisa

Good Excellent 5/5 (Ease of Use)
Abreviews
This is a good Elisa.
The results are good and reproducible.
The protocol booklet is well explained and easy to understand and easy to perform.
I have only used 1 kit for measuring Fas-Ligand so far.
I used this kit for serum samples from a comparison of healthy donor and atopic patients.
The kit is easy to use and quick compared to other Sandwich Elisa.
Based on the recommendations within the booklet we used a serum dilutions of 1: 100 and these dilutions was within the linear range for all but of the 40 samples.
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Abcam user community

Verified customer

提交于 Apr 08 2015

We would suggest testing a range of dilutions to find a dilution within the standard curve. A good range is neat, 1:5, 1:10, 1:20. In the protocol the suggested dilution for normal serum/plasma: 2-10 fold, again this needs to be optimized.

Unfortunately, the OD standard is never twice the same, therefore, it is so important to run a fresh standard curve for EVERY assay. The OD needs to be measured, it cannot be not calculated.
How to analyze the data is described under section 8, pag...

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The exact epitopes are not known but the antibody pair is binding somewhere on the extracellular domain (aa 134-281). If it helps, the capture antibody is monoclonal and the detection antibody is polyclonal. We do not have a kit against HA although we ...

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I can confirm that these kits will detect both soluble and membrane bound Fas and fas ligand.

In order to prevent membrane bound protein to interfere with your results, I suggest to spin down the saliva samples to get rid of all cells while t...

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I have reviewed the details on the datasheets for these kits. For the Fas ligand kits:

ab45892 Fas Ligand Human ELISA Kit
ab100515 Fas Ligand Human ELISA Kit

Sensitivities are different:ab100515 < 2 pg/ml, ab45892 < 12 pg/ml ...

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These ELISA kits are from two different sources but they are similar.

I recommend to determine which is the best for your needs from the datasheet and protocol information. Please take not of the different ranges these kits are suitable for. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"