人Factor XIII ELISA试剂盒(ab108836)

概述

  • 产品名称
    人Factor XIII ELISA试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.5%
    批次间
    样品 n Mean SD CV%
    Overall 8.7%
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 2.1 ng/ml
  • 范围
    5 ng/ml - 80 ng/ml
  • 复检

    99 %

  • 检测时间
    4h 0m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Factor XIII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of factor XIII in plasma, serum, milk, urine, saliva, and cell culture supernatants.

    A Factor XIII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XIII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XIII captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Factor XIII Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Factor XIII Microplate (12 x 8 well strips) 1 unit
    Factor XIII Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究领域
  • 功能
    Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
  • 疾病相关
    Factor XIII subunit A deficiency
  • 序列相似性
    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • 翻译后修饰
    The activation peptide is released by thrombin.
  • 细胞定位
    Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
  • Information by UniProt
  • 别名
    • Coagulation factor XIII A chain
    • Coagulation factor XIIIa
    • F13A_HUMAN
    • F13a1
    • Protein-glutamine gamma-glutamyltransferase A chain
    • Transglutaminase A chain
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

图片

  • Representative Standard Curve using ab108836

实验方案

文献

ab108836 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us.

The kit using different antibodies, but the binding site is not mapped. Since they both polyclonal antibody, it may have multiple binding sites for protein.


I hope this information is helpful to you. ...

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Thank you for contacting us.


Yes, the kit will detect both the A and B forms.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thanks for your enquiry!

Please contact our bulk order specialist, Andrea Gray at mailto:Andrea.Gray@abcam.com, regarding discounts on bulk order purchases.

I hope this is helpful. Please contact me again if you have any further que...

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Thank you for your call last week and for your patience while I've been in touch with the lab regarding your questions.

I've heard back from my contact at the lab, and she agrees that the OD values are lower than expected. An OD of 1.5-2.0 wo...

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Thank you for your call the other day.

I am happy to confirm that the kit ab108836 against the human Factor XIII will recognize the full length protein as well as the fragment of the 37 first amino acids. I have just received the confirmation...

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This ELISA kit will recognize factor XIIIa and factor XIII.  I hope this is helpful. Please contact me again if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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