人Factor XIII ELISA试剂盒(ab108836)
Key features and details
- Sensitivity: 1.5 ng/ml
- Range: 5 ng/ml - 80 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Factor XIII ELISA试剂盒
参阅全部 Factor XIIIa 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5% 批次间 样品 n Mean SD CV% Overall 9.4% -
样品类型
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Tissue, Cell Lysate, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
= 1.5 ng/ml -
范围
5 ng/ml - 80 ng/ml -
回收率
99 %
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检测时间
4h 0m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Factor XIII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of factor XIII in plasma, serum, milk, urine, saliva, cell culture, and cell lysate samples.
A Factor XIII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XIII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XIII captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Factor XIII Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Factor XIII Microplate (12 x 8 well strips) 1 unit Factor XIII Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. -
疾病相关
Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. -
序列相似性
Belongs to the transglutaminase superfamily. Transglutaminase family. -
翻译后修饰
The activation peptide is released by thrombin. -
细胞定位
Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma. - Information by UniProt
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别名
- bA525O21.1 (coagulation factor XIII, A1 polypeptide)
- Coagulation factor XIII A chain
- Coagulation factor XIII A1 polypeptide
see all -
数据库链接
- Entrez Gene: 2162 Human
- Omim: 134570 Human
- SwissProt: P00488 Human
- Unigene: 335513 Human
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab108836 被引用在 3 文献中.
- Matveeva VG et al. Advantages of Fibrin Polymerization Method without the Use of Exogenous Thrombin for Vascular Tissue Engineering Applications. Biomedicines 10:N/A (2022). PubMed: 35453539
- Peltier J et al. Activation peptide of the coagulation factor XIII (AP-F13A1) as a new biomarker for the screening of colorectal cancer. Clin Proteomics 15:15 (2018). PubMed: 29657559
- Liang Y et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). PubMed: 29599912