人Factor XI ELISA试剂盒(ab108834)

概述

  • 产品名称
    人Factor XI ELISA试剂盒
    参阅全部 Factor XI 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 5%
    批次间
    样品 n Mean SD CV%
    Overall 7%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 1.5 ng/ml
  • 范围
    1.56 ng/ml - 50 ng/ml
  • 复检

    97.5 %

  • 检测时间
    4h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Factor XI Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor XI levels in plasma, serum, and cell culture supernatants.

    A Factor XI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XI captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    70X Biotinylated Human Factor XI Antibody 1 x 90µl
    Chromogen Substrate 1 x 8ml
    Factor XI Microplate (12 x 8 well strips) 1 unit
    Factor XI Standard 2 vials
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究领域
  • 功能
    Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
  • 组织特异性
    Isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells.
  • 疾病相关
    Factor XI deficiency (FA11D) [MIM:612416]: A hemorrhagic disease characterized by reduced levels and activity of factor XI resulting in moderate bleeding symptoms, usually occurring after trauma or surgery. Patients usually do not present spontaneous bleeding but women can present with menorrhagia. Hemorrhages are usually moderate. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • 序列相似性
    Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
    Contains 4 apple domains.
    Contains 1 peptidase S1 domain.
  • 翻译后修饰
    Activated by factor XIIa (or XII), which cleaves each polypeptide after Arg-387 into the light chain, which contains the active site, and the heavy chain, which associates with high molecular weight (HMW) kininogen.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • Coagulation factor XI
    • Coagulation factor XIa light chain
    • F11
    • FA11_HUMAN
    • FXI
    • Plasma thromboplastin antecedent
    • PTA
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

图片

  • Representative Standard Curve using ab108834.

实验方案

文献

ab108834 has not yet been referenced specifically in any publications.

客户评价及客户问答

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1. At the power form, it should be good for around 1 year.
2. After dissolve in buffer, the standard need to be aliquot and store at -80 degree if possible. If store at -80, the standard can be good for at le...

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Thank you for contacting us.

The plate can last for at least one year from the date received the kit as long as the pouch un-open. If the pouch has been opened, please keep it in the desicator if you have one. Keep dry is very important. If y...

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Comme indiqué dans le protocol du kit ELISA facteur XI ab108834 (http://www.abcam.com/ps/products/108/ab108834/documents/ab108834%20Factor%20XI%20Human%20ELISA_v3%20%28Website%29.pdf), il est conseillé d'effectuer 2 ou 3 séries de 7 points de standards...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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