人Factor X ELISA试剂盒(ab108832)

概述

  • 产品名称人Factor X ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.8%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 1.5 ng/ml
  • 范围
    1.563 ng/ml - 100 ng/ml
  • 复检

    97.5 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Factor X Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor X concentrations in plasma, serum, milk, urine, saliva and cell culture supernatants.

    A Factor X specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor X specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor X captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
  • 组织特异性Plasma; synthesized in the liver.
  • 疾病相关Factor X deficiency
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • 翻译后修饰The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
    The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Activated factor Xa heavy chain
    • F10
    • FA10_HUMAN
    • Stuart factor
    • Stuart-Prower factor
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Factor X ELISA Kit 图像

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Factor X measured in undiluted culture supernatants with MCF7 levels below level of detection (duplicates +/- SD).

  • Factor X measured in biological fluids (duplicates +/- SD). Serum and plasma were tested at 1/300-1/3000; other samples at 1/1-1/30.

  • Representative Standard Curve using ab108832

实验方案

Human Factor X ELISA Kit (ab108832)参考文献

ab108832 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and your interest in our products.

The Factor X Human ELISA kit, ab108832 enables both FX and FXa to detected in a range of biological samples.

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Thank you for alerting us to this mistake in the protocol.

I can confirm that the biotinylated antibody (page 10, step 5) should be incubated for 1 hour.

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ab108832 may be storedfor up tosix monthat 2 to 8°C or -20°C from the date of shipment.

Thiskits as all our products is covered by our Abpromiseguarantee for six month afterdate of purchase:
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"