人Factor X ELISA试剂盒(ab108832)

概述

  • 产品名称
    人Factor X ELISA试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.8%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 1.5 ng/ml
  • 范围
    1.563 ng/ml - 100 ng/ml
  • 复检

    97.5 %

  • 检测时间
    4h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Factor X Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor X concentrations in plasma, serum, milk, urine, saliva and cell culture supernatants.

    A Factor X specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor X specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor X captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    Biotinylated antibody (50x) 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Factor X Microplate (12 x 8 well strips) 1 unit
    Factor X Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究领域
  • 功能
    Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
  • 组织特异性
    Plasma; synthesized in the liver.
  • 疾病相关
    Factor X deficiency
  • 序列相似性
    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • 翻译后修饰
    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
    The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • Activated factor Xa heavy chain
    • F10
    • FA10_HUMAN
    • Stuart factor
    • Stuart-Prower factor
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

图片

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Factor X measured in undiluted culture supernatants with MCF7 levels below level of detection (duplicates +/- SD).

  • Factor X measured in biological fluids (duplicates +/- SD). Serum and plasma were tested at 1/300-1/3000; other samples at 1/1-1/30.

  • Representative Standard Curve using ab108832

实验方案

文献

ab108832 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us and your interest in our products.

The Factor X Human ELISA kit, ab108832 enables both FX and FXa to detected in a range of biological samples.

I hope this information has been helpful. If you have any furt...

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Thank you for alerting us to this mistake in the protocol.

I can confirm that the biotinylated antibody (page 10, step 5) should be incubated for 1 hour.

The protocol will be up-dated as soon as possible.

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Thank you for your inquiry.

ab108832 may be storedfor up tosix monthat 2 to 8°C or -20°C from the date of shipment.

Thiskits as all our products is covered by our Abpromiseguarantee for six month afterdate of purchase:
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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