人Factor VIII exon 1 ChIP引物and Probe Set (ab85775)

概述

  • 产品名称人Factor VIII exon 1 ChIP引物and Probe Set
    参阅全部 Factor VIII 试剂盒
  • 产品概述

    Accession number: M14113.
    The HsF8 primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells.

    F8 (Coagulation factor VIII) is expressed in liver but silenced in most other cell types. The primers and probe are located in exon 1. In most cell types F8 is enriched with histone modifications associated with silencing (e.g. Histone H3 K27 di methylation, see image with ab24684). Whereas it shows low levels of histone modifications and factors associated with active gene transcription (e.g. RNA polymerase II CTD repeat, see image with ab26721).

性能

  • 功能Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
  • 疾病相关Defects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.
  • 序列相似性Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 2 F5/8 type C domains.
    Contains 6 plastocyanin-like domains.
  • 结构域Domain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity.
  • 翻译后修饰Sulfation on Tyr-1699 is essential for binding vWF.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 别名
    • AHF
    • Antihemophilic factor
    • Coagulation factor VIII
    • coagulation factor VIII, procoagulant component
    • coagulation factor VIIIc
    • DXS1253E
    • F8
    • F8b
    • F8c
    • FA8_HUMAN
    • factor VIII F8B
    • Factor VIIIa light chain
    • FactorVIII
    • FVIII
    • Hema
    • Hemophilia A
    • Hemophilia, classic
    • OTTHUMP00000061446
    • OTTHUMP00000196174
    • Procoagulant component
    see all

Human Factor VIII exon 1 ChIP Primer and Probe Set (ab85775)参考文献

ab85775 has not yet been referenced specifically in any publications.

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We have tested some of our RNA polymerase II antibodies against both inactive genes and the 3' end of active genes. Below is a link to the antibodies.

http://www.abcam.com/RNA-polymerase-II-CTD-repeat-...

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