人Factor D ELISA试剂盒(ab99969)

概述

  • 产品名称人Factor D ELISA试剂盒
    参阅全部 Factor D 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 4 pg/ml
  • 复检

    < 130 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 126.4 115% - 133%
    Serum 130.3 118% - 139%
    Plasma 131.1 107% - 140%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human Factor D ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Factor D in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Factor D coated on a 96-well plate. Standards and samples are pipetted into the wells and Factor D present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Factor D antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Factor D bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
  • 疾病相关Complement factor D deficiency (CFDD) [MIM:613912]: An immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Adipsin
    • ADN
    • C3 convertase activator
    • CFAD_HUMAN
    • CFD
    • Complement factor D
    • Complement factor D preproprotein
    • D component of complement
    • DF
    • PFD
    • Properdin factor D
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab99969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Factor D ELISA Kit 图像

  • Representative Standard Curve using ab99969.

  • Representative Standard Curve using ab99969.

实验方案

Human Factor D ELISA Kit (ab99969)参考文献

ab99969 has not yet been referenced specifically in any publications.

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