人Estradiol E2 ELISA试剂盒(ab108640)

概述

  • 产品名称人Estradiol E2 ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    13 pg/mL 24 3 24.1%
    73 pg/mL 24 7 10.3%
    247 pg/mL 24 10 4.1%
    633 pg/mL 24 31 4.9%
    批次间
    样品 n Mean SD CV%
    14 pg/mL 20 4 26.7%
    82 pg/mL 20 8 10.3%
    264 pg/mL 20 17 6.4%
    691 pg/mL 20 45 6.6%
  • 测试
    1 x 96 well plate
  • 样品类型
    Serum
  • 检测类型Competitive
  • 灵敏度
    10 pg/ml
  • 范围
    10 pg/ml - 1000 pg/ml
  • 复检

    101.3 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 101.3 18pg/ml - 810pg/ml

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Estradiol E2 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Estradiol E2 in Human serum.

    A 96-well plate has been pre-coated with goat anti-rabbit IgG. Samples, standards and the rabbit anti-Estradiol E2 Reagent and Estradiol E2-HRP conjugate are added to the wells, where Estradiol E2 in the sample competes with the added Estradiol E2-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol E2 in the sample and the intensity is measured at 450 nm.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

    应用

    Our Abpromise guarantee covers the use of ab108640 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Competitive ELISA Use at an assay dependent concentration.

    Human Estradiol E2 ELISA Kit 图像

    • Representative standard curve using ab108640

    实验方案

    Human Estradiol E2 ELISA Kit (ab108640)参考文献

    ab108640 has not yet been referenced specifically in any publications.

    Product Wall

    ab108640 reviews

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    We purchased this ELISA kit to screen aromatase inhibitor using cultured MCF-7 cells (ER-positive breast cancer cell line). Good amount of reagents are provided in easy-to-handle vials. Good data obtained with both trials.
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    Abcam user community

    Verified customer

    提交于 Dec 17 2014

    Thank you for your enquiry. I am sorry to confirm that as far as we are aware, these kits have not been tested with samples from rat. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these are updated ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"