概述
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产品名称人Estradiol E2 ELISA试剂盒
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检测方法Colorimetric
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精确度
批次内 样品 n Mean SD CV% 13 pg/mL 24 3 24.1% 73 pg/mL 24 7 10.3% 247 pg/mL 24 10 4.1% 633 pg/mL 24 31 4.9% 批次间 样品 n Mean SD CV% 14 pg/mL 20 4 26.7% 82 pg/mL 20 8 10.3% 264 pg/mL 20 17 6.4% 691 pg/mL 20 45 6.6% -
样品类型Serum
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检测类型Competitive
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灵敏度10 pg/ml
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范围10 pg/ml - 1000 pg/ml
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回收率
101.3 %
特定样本回收率 样品类型 平均% 范围 Serum 101.3 18pg/ml - 810pg/ml -
实验步骤Multiple steps standard assay
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种属反应性与反应: Human
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产品概述
Abcam’s Estradiol E2 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Estradiol E2 in Human serum.
A 96-well plate has been pre-coated with goat anti-rabbit IgG. Samples, standards and the rabbit anti-Estradiol E2 Reagent and Estradiol E2-HRP conjugate are added to the wells, where Estradiol E2 in the sample competes with the added Estradiol E2-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol E2 in the sample and the intensity is measured at 450 nm.
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经测试应用适用于: Competitive ELISAmore details
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平台Microplate
性能
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存放说明Store at +4°C. Please refer to protocols.
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组件 标识符 1 x 96 tests Estradiol E2 Control 1 - 13.9pg/ml 1 x 0.5ml Estradiol E2 Control 2 - 269 pg/ml 1 x 0.5ml Estradiol E2 Standard 1 - 0 pg/mL 1 x 0.5ml Estradiol E2 Standard 2 - 10 pg/mL 1 x 0.5ml Estradiol E2 Standard 3 - 30 pg/mL 1 x 0.5ml Estradiol E2 Standard 4 - 100 pg/mL 1 x 0.5ml Estradiol E2 Standard 5 - 300 pg/mL 1 x 0.5ml Estradiol E2 Standard 6 - 1000 pg/mL 1 x 0.5ml Estradiol E2-HRP Conjugate Reagent Blue colour 1 x 12ml Goat anti-rabbit IgG Coated Microplate (12 x 8 wells) 1 x 96 tests Rabbit Anti-Estradiol E2 Reagent Pink colour 1 x 7ml Stop Solution 1 x 11ml TMB Substrate Solution 1 x 11ml -
研究领域
应用
Our Abpromise guarantee covers the use of ab108640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA | Use at an assay dependent concentration. |
图片
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Typical Standard CurveRepresentative standard curve using ab108640
实验方案
文献
ab108640 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"