人Erythropoietin ELISA试剂盒(EPO) (ab119522)

概述

  • 产品名称
    人Erythropoietin ELISA试剂盒(EPO)
    参阅全部 Erythropoietin (EPO) 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8 3.4%
    批次间
    样品 n Mean SD CV%
    Overall 8 6.2%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    0.4 mIU/ml
  • 范围
    1.6 mIU/ml - 100 mIU/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Serum 81 71% - 93%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Erythropoietin (EPO) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Erythropoietin concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

    Erythropoietin specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated Erythropoietin detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and unbound conjugates are then washed away using was buffer. TMB is then added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Erythropoietin captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab119522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Human Erythropoietin standard curve: mean of duplicates (+/- SD)
  • Human Erythropoietin measured in biological fluids showing quantity (mIU) per mL of tested sample. Samples diluted 1-4 fold.

  • Representative Standard Curve using ab119522.

实验方案

文献

This product has been referenced in:
  • Hansen HG  et al. Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells. Sci Rep 5:18016 (2015). WB . Read more (PubMed: 26657798) »

See 1 Publication for this product

客户评价及客户问答

Thank you for your enquiry regarding ab119522.

This product has been designed to detect human Erythropoietin (EPO) and it has only been tested on human samples; we do not have any information regarding its cross-reactivity with other species....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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