人ErbB3 ELISA试剂盒(ab100511)

概述

  • 产品名称
    人ErbB3 ELISA试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 4 pg/ml
  • 范围
    3.43 pg/ml - 2500 pg/ml
  • 复检

    > 90 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 100.1 78% - 113%
    Serum 95.12 79% - 111%
    Plasma 88.87 71% - 106%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s ErbB3 (Epidermal Factor Growth Factor Receptor 3) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ErbB3 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for human ErbB3 coated on a 96-well plate. Standards and samples are pipetted into the wells and ErbB3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ErbB3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ErbB3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab100511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • TYPICAL STANDARD CURVE - Assay Diluent A – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
  • TYPICAL STANDARD CURVE - Assay Dilent B – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

实验方案

文献

ab100511 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your inquiry and sending that information. I went ahead and plotted the data using a linear regression model and it looks beautiful with strong signal strength, low duplicate CV, and nice linearity (R2 > 0.99). See attachment. The manu...

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Thank you for contacting Abcam. It was a pleasure speaking with you. I have a response from the lab to the the questions that you had brought up yesterday. In regards to whether the antibody works against the free or bound forms of ErbB3, the ELH-ErbB3...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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