人Eotaxin 2 ELISA试剂盒(CCL24) (ab100509)

概述

  • 产品名称人Eotaxin 2 ELISA试剂盒(CCL24)
    参阅全部 Eotaxin 2 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 2 pg/ml
  • 范围
    1.37 pg/ml - 1000 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.77 82% - 103%
    Serum 94.86 83% - 103%
    Plasma 96.74 85% - 104%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Eotaxin 2 (CCL24) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Eotaxin 2 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Eotaxin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Eotaxin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Eotaxin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Eotaxin 2 is a member of the CC chemokine family, based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin 2 cDNA encodes a 119 amino acid residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N glycosylation site. Mature human eotaxin 2 has a predicted molecular mass of approximately 10.6 kDa. Compared to other CC chemokines, eotaxin 2 exhibits 40 %, 42 %, and 39 % amino acid identity to MCP3, MIP1 alpha, and eotaxin, respectively. Human CC chemokine eotaxin 2 maps to chromosome 7q11.23. Both eotaxin and eotaxin 2 activate and attract eosinophils and basophils. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3. On eosinophils, the effects of eotaxin 2 is inhibited by an CCR3 antibody and cross-desensitized by eotaxin and MCP4, suggesting that all three CC chemokines act through CCR3. Eotaxin 2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant eotaxin 2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin 2 inhibits colony formation in myleloid progenitor cells.
  • 细胞定位Secreted
  • 别名
    • C C motif chemokine 24
    • CCL24
    • Chemokine (C C motif) ligand 24
    • Chemokine CC Motif Ligand 2
    • CK beta 6
    • Ckb 6
    • Ckb6
    • Eosinophil chemotactic protein 2
    • Eotaxin 2 like protein
    • MPIF 2
    • MPIF2
    • Myeloid progenitor inhibitory factor 2
    • OTTHUMP00000210225
    • SCYA24
    • Small inducible cytokine A24
    • Small inducible cytokine subfamily A (Cys Cys) member 24
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Eotaxin 2 ELISA Kit (CCL24) 图像

  • Representative Standard Curve using ab100509.

  • Representative Standard Curve using ab100509.

实验方案

Human Eotaxin 2 ELISA Kit (CCL24) (ab100509)参考文献

ab100509 has not yet been referenced specifically in any publications.

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