人Anti-Entamoeba histolytica IgG ELISA试剂盒(ab108734)

概述

  • 产品名称人Anti-Entamoeba histolytica IgG ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Pos.Serum 7 2.1%
    批次间
    样品 n Mean SD CV%
    Pos.Serum 6 3.9%
  • 测试
    1 x 96 test
  • 样品类型
    Serum, Plasma
  • 检测类型Indirect
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s anti-Entamoeba histolytica IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Entamoeba histolytica in Human serum and plasma.

     

    A 96-well plate has been precoated with Entamoeba histolytica antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a Protein A peroxidase labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Entamoeba histolytica-specific antibodies. TMB is then catalyzed by the Protein A to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Entamoeba histolytica IgG sample captured in plate.

  • 经测试应用适用于: Indirect ELISAmore details
  • 平台Microplate

性能

  • 存放说明Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests
    20X Washing Solution White cap 1 x 50ml
    Cover foil 1 unit
    Entamoeba histolytica (IgG) Cut-off Control colored yellow; green cap 1 x 3ml
    Entamoeba histolytica (IgG) Negative Control colored yellow; blue cap 1 x 2ml
    Entamoeba histolytica (IgG) Positive Control colored yellow; red cap 1 x 2ml
    Entamoeba histolytica Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit
    Entamoeba histolytica Protein A Conjugate colored blue; black cap 1 x 20ml
    IgG Sample Diluent colored yellow; white cap 1 x 100ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution Yellow cap 1 x 15ml
  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab108734 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Indirect ELISA Use at an assay dependent dilution.

    实验方案

    Human Anti-Entamoeba histolytica IgG ELISA Kit (ab108734)参考文献

    ab108734 has not yet been referenced specifically in any publications.

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