人Endostatin ELISA试剂盒(ab100508)

概述

  • 产品名称人Endostatin ELISA试剂盒
    参阅全部 Endostatin 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    13.7 pg/ml - 10000 pg/ml
  • 复检

    > 90 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.32 84% - 108%
    Serum 88.47 78% - 102%
    Plasma 92.31 80% - 104%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human Endostatin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Endostatin in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Endostatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Endostatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Endostatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Endostatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Endostatin (20-22 kDa), a cleaved product of the C-terminal domain of collagen XVIII, is an endogenous angiogenesis inhibitor. It was originally found in conditioned media from a murine endothelial tumor cell line, hemangioendothelioma. Endostatin inhibits endothelial cell migration (in vivo and in vitro) and induces endothelial cell apoptosis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes.
  • 别名
    • Alpha 1 collagen type 18 (XVIII)(COL18A1)
    • Alpha 1 type XVIII collagen
    • Antiangiogenic agent
    • COL18A1
    • Collagen alpha 1(XVIII) chain
    • Collagen type XVIII alpha 1
    • FLJ27325
    • FLJ34914
    • KNO
    • KNO1
    • MGC74745
    • Multi functional protein MFP
    • OTTMUSP00000023764
    • OTTMUSP00000023766
    • Procollagen type XVIII alpha 1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Endostatin ELISA Kit 图像

  • Representative Standard Curve using ab100508

  • Representative Standard Curve using ab100508

实验方案

Human Endostatin ELISA Kit (ab100508)参考文献

ab100508 has not yet been referenced specifically in any publications.

Product Wall

Both diluents should be clear. Salts, proteins, and/or other solutes may precipitate out after long periods of storage but bringing the solution up to room temperature and mixing well should re-solubilize any aggregates.


In regards to y...

Read More


It is not necessary to dilute samples - especially if the endostatin levels are already very low. The main purpose of diluting your samples is to make sure that your sample OD falls within the OD range of your standard curve so you can accurately ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"