人Elafin ELISA试剂盒(Skalp) (ab100658)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 6.86 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Elafin ELISA试剂盒(Skalp)
参阅全部 Elafin/ESI 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 5 pg/ml -
范围
6.86 pg/ml - 5000 pg/ml -
回收率
> 100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 111.8 102% - 121% Serum 115.7 105% - 125% Plasma 112.5 103% - 122% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Elafin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Elafin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Elafin coated on a 96-well plate. Standards and samples are pipetted into the wells and Elafin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Elafin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Elafin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-Human Elafin 2 vials Elafin Microplate (12 x 8 wells) 1 unit Recombinant Human Elafin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. -
序列相似性
Contains 1 WAP domain. -
结构域
Consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins. -
细胞定位
Secreted. - Information by UniProt
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别名
- Cementoin
- ELAF_HUMAN
- Elafin
see all -
数据库链接
- Entrez Gene: 5266 Human
- Omim: 182257 Human
- SwissProt: P19957 Human
- Unigene: 112341 Human
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab100658 被引用在 3 文献中.
- Olmos-Ortiz A et al. Compartmentalized Innate Immune Response of Human Fetal Membranes against Escherichia coli Choriodecidual Infection. Int J Mol Sci 23:N/A (2022). PubMed: 35328414
- Tehrani FA et al. Induction of antimicrobial peptides secretion by IL-1ß enhances human amniotic membrane for regenerative medicine. Sci Rep 7:17022 (2017). ELISA . PubMed: 29208979
- Tehrani FA et al. The effects of preservation procedures on antibacterial property of amniotic membrane. Cryobiology 67:293-8 (2013). PubMed: 23988559