人EGFR ELISA试剂盒(ERBB) (ab100505)

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概述

  • 产品名称
    人EGFR ELISA试剂盒(ERBB)
    参阅全部 EGFR 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 4 pg/ml
  • 范围
    2.74 pg/ml - 2000 pg/ml
  • 回收率

    > 85 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 85.65 72% - 96%
    Serum 105.4 94% - 116%
    Plasma 107.2 96% - 119%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s EGFR (ERBB) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human EGFR in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human EGFR coated on a 96-well plate. Standards and samples are pipetted into the wells and EGFR present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman EGFR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGFR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Human soluble EGFR ELISA Kit (ab193764) from our SimpleStep ELISA® range.


     

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human EGFR 2 vials
    EGFR Microplate (12 x 8 well strips) 1 unit
    Recombinant Human EGFR Standard 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • 组织特异性
    Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • 疾病相关
    Defects in EGFR are associated with lung cancer (LNCR) [MIM:211980].
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.
  • 细胞定位
    Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Co-localizes with TNK2 on the endosomes.
  • Information by UniProt
  • 别名
    • 9030024J15Rik
    • AI552599
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog
    • Avian erythroblastic leukemia viral (verbb) oncogene homolog
    • Cell growth inhibiting protein 40
    • Cell proliferation inducing protein 61
    • EGF R
    • EGFR
    • EGFR_HUMAN
    • Epidermal growth factor receptor
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog)
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian)
    • Erbb
    • erbb 1
    • Erbb1
    • Erythroblastic leukemia viral (v erb-b) oncogene homolog
    • HER1
    • mENA
    • Oncogene ERBB
    • OTTMUSP00000005385
    • OTTMUSP00000005386
    • PIG61
    • Proto oncogene c ErbB 1
    • Proto-oncogene c-ErbB-1
    • Receptor tyrosine protein kinase ErbB 1
    • Receptor tyrosine protein kinase ErbB1
    • Receptor tyrosine-protein kinase ErbB-1
    • RP23-295E4.1
    • Urogastrone
    • wa2
    • Wa5
    see all
  • 数据库链接

    相关产品

    应用

    Our Abpromise guarantee covers the use of ab100505 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Sandwich ELISA Use at an assay dependent concentration.

    图片

    • Sandwich ELISA - ab100505 EGFR (ERBB) Human ELISA Kit
      Sandwich ELISA - ab100505 EGFR (ERBB) Human ELISA Kit

      EGFR measured in cell lysates showing quantity (ng) per 1 million cells

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100505.

    • Sandwich ELISA - ab100505 EGFR (ERBB) Human ELISA Kit
      Sandwich ELISA - ab100505 EGFR (ERBB) Human ELISA Kit

      EGFR measured in various fluids showing quantity (ng) per mL of tested sample

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100505.

    实验方案

    文献

    ab100505 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab100505？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    4 reviews or Q&A

    10
    Votes: Highest First

    Hi there. I was wondering if your elisa kit (ab 100505) can measure EGFR ligands in media?

    Thank you for your inquiry.

    Unfortunately we haven't tested ab100505 for its cross-reactivity with EGFR ligands such as EGF, Amphiregulin, STAT1, etc. However, we would not think that EGFR and its ligands would be very similar so we would not...

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    I see there is a recombinant standard included with the kit. Is this protein composed of the inracellular or extracellular region of the protein?

    This protein contains the extracellular region of EGFR.

    Inquiry: Dear sir/Madam, I have recently bought a purified recombinant protein (EGFR, ycp1031) to Speed biosystems who have used some components of your EGFR Human ELISA Kit (ab100505) to test for EGFR-EGF binding. In particular, they have used the &qu...

    Read More

    Thank you for your enquiry.

    I can confirm that this particular kit is sourced externally and the constituents of this buffer are considered proprietary (commercially sensitive). I am sorry we are therefore regrettably not able to provide any...

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    We are planning to analyse EGFR and P53 by blood serum of lung cancer patient with Elisa. My query regarding this is whether you cater to these kind of kits? If yes what will be the amount for 100 patients? We had done EGFR and p53 earlier with IHC on ...

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    Thank you for contacting us.

    We have two P53 related kits in our catalogue ab46067 and ab46113. These kids have been tested and fully guaranteed to be used with human serum samples. In any case if these does not work we will be happy to provi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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