概述

  • 产品名称
    人EG-VEGF ELISA试剂盒(PK1)
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 7 pg/ml
  • 范围
    15.6 pg/ml - 1000 pg/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human EG-VEGF (PK1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human EG-VEGF (PK1) in cell culture supernatants and plasma (heparin, EDTA).


    A EG-VEGF (PK1) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for EG-VEGF (PK1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human EG-VEGF (PK1) amount of sample captured in plate.

  • 说明

    This kit may not be sensitive enough to detect EG-VEGF in serum samples and this sample type is not covered by our AbPromise.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    ABC Diluent Buffer 1 x 12ml
    Antibody Diluent Buffer 1 x 12ml
    Anti-Human EG-VEGF antibody Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human EG-VEGF antibody 1 x 130µl
    Lyophilized recombinant Human EG-VEGF standard 2 x 10ng
    Plate Seal 1 x 4 units
    Sample Diluent Buffer 1 x 30ml
    TMB Color Developing Agent 1 x 10ml
    TMB Stop Solution 1 x 10ml
  • 研究领域
  • 功能
    Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis.
  • 组织特异性
    Localizes to glandular epithelium, stroma and vascular epithelial cells of first trimester decidua (at protein level). Up-regulated in first trimester decidua when compared with non-pregnant endometrium. Expressed in the steroidogenic glands, ovary, testis, adrenal and placenta.
  • 序列相似性
    Belongs to the AVIT (prokineticin) family.
  • 发展阶段
    In adult testis, is strongly expressed only in Leydig cells. In testicular tumors, expressed specifically in Leydig cell tumors (at protein level). Expressed from 14 weeks until birth in fetal testis.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • EG-VEGF
    • Endocrine-gland-derived vascular endothelial growth factor
    • Mambakine
    • PROK1
    • PROK1_HUMAN
    • Prokineticin-1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119591.

实验方案

文献

ab119591 has not yet been referenced specifically in any publications.

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