人EG-VEGF ELISA试剂盒(PK1) (ab119591)
Key features and details
- Sensitivity: 7 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人EG-VEGF ELISA试剂盒(PK1) -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 7 pg/ml -
范围
15.6 pg/ml - 1000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human EG-VEGF (PK1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human EG-VEGF (PK1) in cell culture supernatants and plasma (heparin, EDTA).
A EG-VEGF (PK1) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for EG-VEGF (PK1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human EG-VEGF (PK1) amount of sample captured in plate.
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说明
This kit may not be sensitive enough to detect EG-VEGF in serum samples and this sample type is not covered by our AbPromise.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human EG-VEGF antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Human EG-VEGF antibody 1 x 100µl Lyophilized recombinant Human EG-VEGF standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究领域
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功能
Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis. -
组织特异性
Localizes to glandular epithelium, stroma and vascular epithelial cells of first trimester decidua (at protein level). Up-regulated in first trimester decidua when compared with non-pregnant endometrium. Expressed in the steroidogenic glands, ovary, testis, adrenal and placenta. -
序列相似性
Belongs to the AVIT (prokineticin) family. -
发展阶段
In adult testis, is strongly expressed only in Leydig cells. In testicular tumors, expressed specifically in Leydig cell tumors (at protein level). Expressed from 14 weeks until birth in fetal testis. -
细胞定位
Secreted. - Information by UniProt
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别名
- Black mamba toxin related protein
- EG VEGF
- EG-VEGF
see all -
数据库链接
- Entrez Gene: 84432 Human
- Omim: 606233 Human
- SwissProt: P58294 Human
- Unigene: 514793 Human
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab119591 尚未被引用在任何文献中。