概述

  • 产品名称人EG-VEGF ELISA试剂盒(PK1)
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 7 pg/ml
  • 范围
    15.6 pg/ml - 1000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human EG-VEGF (PK1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human EG-VEGF (PK1) in cell culture supernatants, serum and plasma (heparin, EDTA).

    A EG-VEGF (PK1) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for EG-VEGF (PK1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human EG-VEGF (PK1) amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis.
  • 组织特异性Localizes to glandular epithelium, stroma and vascular epithelial cells of first trimester decidua (at protein level). Up-regulated in first trimester decidua when compared with non-pregnant endometrium. Expressed in the steroidogenic glands, ovary, testis, adrenal and placenta.
  • 序列相似性Belongs to the AVIT (prokineticin) family.
  • 发展阶段In adult testis, is strongly expressed only in Leydig cells. In testicular tumors, expressed specifically in Leydig cell tumors (at protein level). Expressed from 14 weeks until birth in fetal testis.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • EG-VEGF
    • Endocrine-gland-derived vascular endothelial growth factor
    • Mambakine
    • PROK1
    • PROK1_HUMAN
    • Prokineticin-1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human EG-VEGF ELISA Kit (PK1) 图像

  • Representative Standard Curve using ab119591

实验方案

Human EG-VEGF ELISA Kit (PK1) (ab119591)参考文献

ab119591 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119591.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"