概述

描述

  • 性质
    Synthetic

相关产品

技术指标

Our Abpromise guarantee covers the use of ab41752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Blocking - Blocking peptide for Anti-EAAT2 antibody (ab41621)

  • 纯度
    > 90 % n/a.

  • 形式
    Lyophilised
  • 补充说明

    Reconstitution instructions:

     -  First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.

    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.

    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.

    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.

    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

常规信息

  • 别名
    • EAA2_HUMAN
    • EAAT2
    • Excitatory amino acid transporter 2
    • Excitotoxic amino acid transporter 2
    • Glial high affinity glutamate transporter
    • GLT 1
    • GLT1
    • Glutamate aspartate transporter II
    • Glutamate transporter 1
    • Glutamate/aspartate transporter II
    • Slc1a2
    • Sodium dependent glutamate aspartate transporter 2
    • Sodium-dependent glutamate/aspartate transporter 2
    • solute carrier family 1 (glial high affinity glutamate transporter), member 2
    • Solute carrier family 1 glial high affinity glutamate transporter member 2
    • Solute carrier family 1 member 2
    see all
  • 功能
    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
  • 序列相似性
    Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
  • 翻译后修饰
    Glycosylated.
  • 细胞定位
    Membrane.
  • Information by UniProt

文献

This product has been referenced in:
  • Potapenko ES  et al. Altered astrocyte glutamate transporter regulation of hypothalamic neurosecretory neurons in heart failure rats. Am J Physiol Regul Integr Comp Physiol 303:R291-300 (2012). Read more (PubMed: 22696576) »

See 1 Publication for this product

客户评价及客户问答

Application
Other
Predicted band size would be 62 kDa.
however observed band size for cow liver is less then 62 kDa.
Loading amount of each lane : 60ug of cow liver
1 lane: Fresh Primary antibody (ab41621)
2 lane: Fresh Primary antibody with 45 min incubation at RT
3 lane: only Secondary antibody (without primary antibody)
4 lane: Primary antibody : Peptide = 1 : 0.01with 45 min incubation at RT
5 lane: Primary antibody : Peptide = 1 : 0.001with 45 min incubation at RT
6 lane: Primary antibody : Peptide = 1 : 0.0001with 45 min incubation at RT
Username

Mr. Kwangwon Son

Verified customer

提交于 Nov 16 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册