人DR4 ELISA试剂盒(CD261) (ab46510)

概述

  • 产品名称人DR4 ELISA试剂盒(CD261)
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 8 475pg/ml 13 2.76%
    B 8 54pg/ml 4 7.57%
    批次间
    样品 n Mean SD CV%
    A 12 498pg/ml 40 8.11%
    B 12 53pg/ml 7 13.87%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 8 pg/ml
  • 范围
    31.25 pg/ml - 1000 pg/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Serum 93.4 250pg/ml - 1000pg/ml

  • 检测时间
    2h 30m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s DR4 (CD261) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DR4 in serum, plasma, buffered solutions and cell culture media.

    A monoclonal antibody specific for DR4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DR4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DR4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DR4 present in the samples.

    This kit will recognize both endogenous and recombinant Human DR4.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Human Trail R1, CD261 or DR4 (Death Receptor 4) is a TNF receptor, which is a receptor for Trail (APO2 ligand). DR4 is involved in apoptosis. In the Trail receptor family, DR4, but also Trail R2 (DR5) transduce an apoptosis signal, whereas Trail R3 (DcR1) and Trail R4 (DcR2) antagonize TRAIL-induced apoptosis. Binding of trimeric Trail to DR4 induces apoptosis by oligomerization (likely to be trimerization) of the receptor. DR4 cDNA encodes a 468 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. DR4 shares 55% of its amino acid sequence with Trail R2. DR4 is widely expressed in normal tissue.
  • 细胞定位Cell Membrane; single-pass type I membrane protein
  • 别名
    • APO2
    • CD261
    • Death receptor 4
    • TNFRSF10A
    • TRAIL receptor 1
    • TRAILR1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human DR4 ELISA Kit (CD261) 图像

  • Representative Standard Curve using ab46510

实验方案

Human DR4 ELISA Kit (CD261) (ab46510)参考文献

ab46510 has not yet been referenced specifically in any publications.

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A standard lysis buffer (such as RIPA buffer) can be used to prepare the cell lysates and the use protease inhibitor is indeed recommended.

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