人DR4 ELISA试剂盒(CD261) (ab46510)
Key features and details
- Sensitivity: 8 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
-
产品名称
人DR4 ELISA试剂盒(CD261) -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% A 8 475pg/ml 13 2.76% B 8 54pg/ml 4 7.57% 批次间 样品 n Mean SD CV% A 12 498pg/ml 40 8.11% B 12 53pg/ml 7 13.87% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 8 pg/ml -
范围
31.25 pg/ml - 1000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 93.4 250pg/ml - 1000pg/ml -
检测时间
2h 30m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s DR4 (CD261) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DR4 in serum, plasma, buffered solutions and cell culture media.
A monoclonal antibody specific for DR4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DR4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DR4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DR4 present in the samples.
This kit will recognize both endogenous and recombinant Human DR4.
-
说明
The standard is affinity purified.
-
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml Biotinylated anti-DR4 Red 2 x 400µl 1 x 400µl Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml DR4 Microplate (12 x 8 well strips) 2 units 1 unit DR4 Standard (lyophilized) Yellow 4 vials 2 vials HRP Diluent Red 1 x 23ml 1 x 12ml Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl -
研究领域
-
相关性
Human Trail R1, CD261 or DR4 (Death Receptor 4) is a TNF receptor, which is a receptor for Trail (APO2 ligand). DR4 is involved in apoptosis. In the Trail receptor family, DR4, but also Trail R2 (DR5) transduce an apoptosis signal, whereas Trail R3 (DcR1) and Trail R4 (DcR2) antagonize TRAIL-induced apoptosis. Binding of trimeric Trail to DR4 induces apoptosis by oligomerization (likely to be trimerization) of the receptor. DR4 cDNA encodes a 468 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. DR4 shares 55% of its amino acid sequence with Trail R2. DR4 is widely expressed in normal tissue. -
细胞定位
Cell Membrane; single-pass type I membrane protein -
别名
- APO2
- CD261
- Death receptor 4
see all -
数据库链接
- Entrez Gene: 8797 Human
- SwissProt: O00220 Human
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab46510 尚未被引用在任何文献中。