人DKK1 ELISA试剂盒(Dickkopf-1) (ab100501)

概述

  • 产品名称人DKK1 ELISA试剂盒(Dickkopf-1)
    参阅全部 DKK1 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 100 pg/ml
  • 范围
    122.9 pg/ml - 30000 pg/ml
  • 复检

    90 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 108.3 93% - 123%
    Serum 92.3 62% - 130%
    Plasma 72.8 59% - 110%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s DKK1 (Dickkopf-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human DKK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性DKK1 is an inhibitor of the Wnt signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
  • 别名
    • Dickkopf 1
    • Dickkopf homolog 1
    • Dickkopf related protein 1
    • DKK 1
    • hDkk 1
    • hDkk1
    • mDkk1
    • SK
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human DKK1 ELISA Kit (Dickkopf-1) 图像

  • Representative Standard Curve using ab100501.

  • Representative Standard Curve using ab100501.

实验方案

Human DKK1 ELISA Kit (Dickkopf-1) (ab100501)参考文献

ab100501 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your interest in our kits. I will send the product inserts first thing tomorrow morning, EST. The kits will be compatible with the automated ELISA machine, if the automated set-up approximates the conditions specified in the kit protocols...

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This kit has been shown that to cross-react with mouse samples, however, we do not have any information about cross-reactivity with rabbit samples.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"