概述

  • 产品名称人Decorin ELISA试剂盒(DCN)
    参阅全部 Decorin 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 1.5 pg/ml
  • 范围
    0.96 pg/ml - 700 pg/ml
  • 复检

    > 93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 93.82 84% - 104%
    Serum 122.5 111% - 130%
    Plasma 111.8 103% - 119%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Decorin (DCN) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Decorin in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Decorin coated on a 96-well plate. Standards and samples are pipetted into the wells and Decorin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti Human Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Decorin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能May affect the rate of fibrils formation.
  • 疾病相关Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
  • 序列相似性Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
    Contains 12 LRR (leucine-rich) repeats.
  • 翻译后修饰The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 别名
    • Bone proteoglycan II
    • CSCD
    • DCN
    • DCN protein
    • Decorin
    • Decorin proteoglycan
    • Dermatan sulphate proteoglycans II
    • DKFZp686J19238
    • DSPG 2
    • DSPG2
    • PG 40
    • PG II
    • PG S2
    • PG-S2
    • PG40
    • PGII
    • PGS 2
    • PGS2
    • PGS2_HUMAN
    • Proteoglycan core protein
    • SLRR1B
    • Small leucine rich protein 1B
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab99998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Decorin ELISA Kit (DCN) 图像

  • DCN measured in biological fluids showing quantity (pg) per mL of tested sample

  • Representative Standard Curve using ab99998.

实验方案

Human Decorin ELISA Kit (DCN) (ab99998)参考文献

This product has been referenced in:
  • Ono YJ  et al. Decorin induced by progesterone plays a crucial role in suppressing endometriosis. J Endocrinol 223:203-16 (2014). Human . Read more (PubMed: 25244916) »

See 1 Publication for this product

Product Wall

Decorin ELISA kit easy to use

Good Excellent 5/5 (Ease of Use)
Abreviews
It's very easy to use.
We tested this kit on pig wounded skin tissue.
We got acceptable result but we need more samples to statistics analysis.
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提交于 May 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"