概述

  • 产品名称人DCR2 ELISA试剂盒(CD264)
    参阅全部 DcR2 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 8 5723pg/ml 212 3.7%
    Sample B 8 3063pg/ml 218 7.1%
    批次间
    样品 n Mean SD CV%
    Sample A 12 6531pg/ml 627 9.6%
    Sample B 12 3123pg/ml 369 11.8%
  • 测试
    1 x 96 well plate
  • 样品类型
    Serum, Cell culture extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 64 pg/ml
  • 范围
    312.5 pg/ml - 10000 pg/ml
  • 检测时间
    3h 40m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s DCR2 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DCR2 in Human serum, plasma, buffered solutions or supernatants.

    A monoclonal antibody specific for DCR2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DCR2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DCR2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DCR2 present in the samples.

    This kit will recognize both endogenous and recombinant Human DCR2.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性DcR2 is a type 1 TNF R family membrane protein which is a receptor for TRAIL. In the Trail receptor family, DcR2 but also Trail R3 (DcR1) antagonize TRAIL-induced apoptosis whereas Trail R1 (DR4) and Trail R2 (DR5) transducer an apoptosis signal. DcR2 has a truncated intracellular domain. Overexpression of DcR2 has been shown to protect cells bearing Trail R1 and/or Trail R2 from Trail-induced apoptosis. Many tumor cell lines don’t express the decoy receptors and are therefore sensitive to TRAIL if they express Trail R1 and/or Trail R2.
  • 细胞定位Cell Membrane; single-pass type I membrane protein
  • 别名
    • CD264
    • CD264 antigen
    • DCR2
    • Decoy receptor 2
    • TNF related apoptosis inducing ligand receptor 4
    • TRAIL R4
    • TRAIL receptor 4
    • TRAIL receptor with a truncated death domain
    • TRAILR4
    • TRUNDD
    • Tumor necrosis factor receptor superfamily member 10d decoy with truncated death domain
    • Tumor necrosis factor receptor superfamily member 10D precursor
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human DCR2 ELISA Kit (CD264) 图像

  • Representative Standard Curve using ab46017

实验方案

Human DCR2 ELISA Kit (CD264) (ab46017)参考文献

ab46017 has not yet been referenced specifically in any publications.

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