概述

  • 产品名称
    人DCR2 ELISA试剂盒(CD264)
    参阅全部 DcR2 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 8 5723pg/ml 212 3.7%
    Sample B 8 3063pg/ml 218 7.1%
    批次间
    样品 n Mean SD CV%
    Sample A 12 6531pg/ml 627 9.6%
    Sample B 12 3123pg/ml 369 11.8%
  • 样品类型
    Serum, Cell culture extracts
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 64 pg/ml
  • 范围
    312.5 pg/ml - 10000 pg/ml
  • 检测时间
    3h 40m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s DCR2 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DCR2 in Human serum, plasma, buffered solutions or supernatants.

    A monoclonal antibody specific for DCR2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DCR2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DCR2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DCR2 present in the samples.

    This kit will recognize both endogenous and recombinant Human DCR2.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated anti DCR2 Red 1 x 400µl 2 x 400µl
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    DCR2 Microplate (12 x 8 well strips) 1 unit 2 units
    DCR2 Standard (lyophilized) Yellow 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究领域
  • 相关性
    DcR2 is a type 1 TNF R family membrane protein which is a receptor for TRAIL. In the Trail receptor family, DcR2 but also Trail R3 (DcR1) antagonize TRAIL-induced apoptosis whereas Trail R1 (DR4) and Trail R2 (DR5) transducer an apoptosis signal. DcR2 has a truncated intracellular domain. Overexpression of DcR2 has been shown to protect cells bearing Trail R1 and/or Trail R2 from Trail-induced apoptosis. Many tumor cell lines don’t express the decoy receptors and are therefore sensitive to TRAIL if they express Trail R1 and/or Trail R2.
  • 细胞定位
    Cell Membrane; single-pass type I membrane protein
  • 别名
    • CD264
    • CD264 antigen
    • DCR2
    • Decoy receptor 2
    • TNF related apoptosis inducing ligand receptor 4
    • TRAIL R4
    • TRAIL receptor 4
    • TRAIL receptor with a truncated death domain
    • TRAILR4
    • TRUNDD
    • Tumor necrosis factor receptor superfamily member 10d decoy with truncated death domain
    • Tumor necrosis factor receptor superfamily member 10D precursor
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab46017

实验方案

文献

ab46017 has not yet been referenced specifically in any publications.

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