概述

  • 产品名称人DcR2 ELISA试剂盒
    参阅全部 DcR2 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    5.487 pg/ml - 4000 pg/ml
  • 复检

    97 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 98.44 90% - 107%
    Serum 99.99 91% - 107%
    Plasma 93.86 83% - 108%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s DcR2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DcR2 in serum, plasma and cell culture supernatants

    This assay employs an antibody specific for Human DcR2 coated on a 96-well plate. Standards and samples are pipetted into the wells and DcR2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DcR2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DcR2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性DcR2 is a type 1 TNF R family membrane protein which is a receptor for TRAIL. In the Trail receptor family, DcR2 but also Trail R3 (DcR1) antagonize TRAIL-induced apoptosis whereas Trail R1 (DR4) and Trail R2 (DR5) transducer an apoptosis signal. DcR2 has a truncated intracellular domain. Overexpression of DcR2 has been shown to protect cells bearing Trail R1 and/or Trail R2 from Trail-induced apoptosis. Many tumor cell lines don’t express the decoy receptors and are therefore sensitive to TRAIL if they express Trail R1 and/or Trail R2.
  • 细胞定位Cell Membrane; single-pass type I membrane protein
  • 别名
    • CD264
    • CD264 antigen
    • DCR2
    • Decoy receptor 2
    • TNF related apoptosis inducing ligand receptor 4
    • TRAIL R4
    • TRAIL receptor 4
    • TRAIL receptor with a truncated death domain
    • TRAILR4
    • TRUNDD
    • Tumor necrosis factor receptor superfamily member 10d decoy with truncated death domain
    • Tumor necrosis factor receptor superfamily member 10D precursor
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human DcR2 ELISA Kit 图像

  • Representative standard curve using ab100657

  • Representative standard curve using ab100657

实验方案

Human DcR2 ELISA Kit (ab100657)参考文献

ab100657 has not yet been referenced specifically in any publications.

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