概述

  • 产品名称人DcR1 ELISA试剂盒(CD263)
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 8 7171pg/ml 192 2.7%
    Sample B 8 3584pg/ml 244 6.8%
    批次间
    样品 n Mean SD CV%
    Sample A 9 7311pg/ml 515 7%
    Sample B 9 2654pg/ml 356 9.7%
  • 测试
    1 x 96 well plate
  • 样品类型
    Serum, Cell culture extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 147 pg/ml
  • 范围
    312.5 pg/ml - 10000 pg/ml
  • 检测时间
    3h 40m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s DcR1 (CD263) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DcR1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

    A monoclonal antibody specific for DcR1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DcR1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DcR1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DcR1 present in the samples.

    This kit will recognize both endogenous and recombinant Human DcR1.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性DcR1 is a member of the TNF-receptor superfamily. It contains an extracellular TRAIL-binding domain and a transmembrane domain, but no cytoplasmic death domain. It is not capable of inducing apoptosis, and is thought to function as an antagonistic receptor that protects cells from TRAIL-induced apoptosis. This gene was found to be a p53-regulated DNA damage-inducible gene. The expression of this gene was detected in many normal tissues but not in most cancer cell lines, which may explain the specific sensitivity of cancer cells to the apoptosis-inducing activity of TRAIL.
  • 细胞定位Cell membrane; lipid-anchor; GPI-anchor
  • 别名
    • CD263
    • DCR1
    • Decoy receptor 1
    • DR6
    • LIT
    • Lymphocyte inhibitor of TRAIL
    • TRAIL R3
    • TRAIL receptor 3
    • TRAILR3
    • TRID
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human DcR1 ELISA Kit (CD263) 图像

  • Representative Standard Curve using ab46016

实验方案

Human DcR1 ELISA Kit (CD263) (ab46016)参考文献

This product has been referenced in:
  • Anand A  et al. Does DcR1 (TNF-related apoptosis-inducing-ligand Receptor 3) have any role in human AMD pathogenesis? Sci Rep 4:4114 (2014). Read more (PubMed: 24534820) »

See 1 Publication for this product

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