概述

  • 产品名称
    人DcR1 ELISA试剂盒(CD263)
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 8 7171pg/ml 192 2.7%
    Sample B 8 3584pg/ml 244 6.8%
    批次间
    样品 n Mean SD CV%
    Sample A 9 7311pg/ml 515 7%
    Sample B 9 2654pg/ml 356 9.7%
  • 样品类型
    Serum, Cell culture extracts
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 147 pg/ml
  • 范围
    312.5 pg/ml - 10000 pg/ml
  • 检测时间
    3h 40m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s DcR1 (CD263) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DcR1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

    A monoclonal antibody specific for DcR1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DcR1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DcR1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DcR1 present in the samples.

    This kit will recognize both endogenous and recombinant Human DcR1.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-DcR1 Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    DcR1 Microplate (12 x 8 well strips) 1 unit 2 units
    DcR1 Standard (Lyophilized) Yellow 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究领域
  • 相关性
    DcR1 is a member of the TNF-receptor superfamily. It contains an extracellular TRAIL-binding domain and a transmembrane domain, but no cytoplasmic death domain. It is not capable of inducing apoptosis, and is thought to function as an antagonistic receptor that protects cells from TRAIL-induced apoptosis. This gene was found to be a p53-regulated DNA damage-inducible gene. The expression of this gene was detected in many normal tissues but not in most cancer cell lines, which may explain the specific sensitivity of cancer cells to the apoptosis-inducing activity of TRAIL.
  • 细胞定位
    Cell membrane; lipid-anchor; GPI-anchor
  • 别名
    • CD263
    • DCR1
    • Decoy receptor 1
    • DR6
    • LIT
    • Lymphocyte inhibitor of TRAIL
    • TRAIL R3
    • TRAIL receptor 3
    • TRAILR3
    • TRID
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab46016

实验方案

文献

This product has been referenced in:
  • Anand A  et al. Does DcR1 (TNF-related apoptosis-inducing-ligand Receptor 3) have any role in human AMD pathogenesis? Sci Rep 4:4114 (2014). Read more (PubMed: 24534820) »

See 1 Publication for this product

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