人Cytochrome c ELISA试剂盒(ab119521)

概述

  • 产品名称人Cytochrome c ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 6%
    批次间
    样品 n Mean SD CV%
    Overall 4%
  • 测试
    1 x 96 test
  • 样品类型
    Cell Lysate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.05 ng/ml
  • 范围
    0.08 ng/ml - 5 ng/ml
  • 复检

    82 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell Lysate 98 78% - 119%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Cytochrome c Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cytochrome c concentrations in cell lysate.

    Cytochrome c specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated Cytochrome c detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and unbound conjugates are then washed away using was buffer. TMB is then added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cytochrome c captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • 细胞定位Mitochondrion matrix
  • 别名
    • CYC
    • CYCS
    • Cytochrome c somatic
    • HCS
    • THC4
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Cytochrome c ELISA Kit 图像

  • Representative Standard Curve using ab119521.

实验方案

Human Cytochrome c ELISA Kit (ab119521)参考文献

ab119521 has not yet been referenced specifically in any publications.

Product Wall



The lysis buffer in kits ab119507 and ab119521 are identical, so you can analyze lysates prepared for ab119507 with ab119521.
For ab119521, the lysis buffer allows for lysis of the cellular membrane but not other compartments with in the...

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Unfortunately, we would not recommend using the Cytochrome c Human ELISA Kit (with color giving dyes), ab119521 with serum sample as when we have tried this we have observed false pos...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"