概述

  • 产品名称
    人Cytochrome c ELISA试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 6%
    批次间
    样品 n Mean SD CV%
    Overall 4%
  • 样品类型
    Cell Lysate
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    0.05 ng/ml
  • 范围
    0.08 ng/ml - 5 ng/ml
  • 复检

    82 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell Lysate 98 78% - 119%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Cytochrome c Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cytochrome c concentrations in cell lysate.

    Cytochrome c specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated Cytochrome c detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and unbound conjugates are then washed away using was buffer. TMB is then added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cytochrome c captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab119521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119521.

实验方案

文献

This product has been referenced in:
  • Brown AN  et al. Preclinical Evaluations To Identify Optimal Linezolid Regimens for Tuberculosis Therapy. MBio 6:e01741-15 (2015). ELISA ; Human . Read more (PubMed: 26530386) »

See 1 Publication for this product

客户评价及客户问答



The lysis buffer in kits ab119507 and ab119521 are identical, so you can analyze lysates prepared for ab119507 with ab119521.
For ab119521, the lysis buffer allows for lysis of the cellular membrane but not other compartments with in the...

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Thank you for your email and your interest in our products.

Unfortunately, we would not recommend using the Cytochrome c Human ELISA Kit (with color giving dyes), ab119521 with serum sample as when we have tried this we have observed false pos...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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