人CXCL7 ELISA试剂盒(ab100613)

概述

  • 产品名称人CXCL7 ELISA试剂盒
    参阅全部 CXCL7 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Cell Lysate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 8.5 pg/ml
  • 范围
    4.1 pg/ml - 1000 pg/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.28 84% - 103%
    Serum 92.76 82% - 102%
    Plasma 93.45 83% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CXCL7 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL7 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CXCL7 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性CXCL7 is a platelet-derived growth factor that belongs to the CXC chemokine family. It is a potent chemoattractant and activator of neutrophils and has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
  • 别名
    • B TG1
    • Beta TG
    • Beta thromboglobulin
    • C X C motif chemokine 7
    • Connective tissue activating peptide III
    • CTAP III
    • CTAP3
    • CTAPIII
    • LA PF4
    • LDGF
    • Leukocyte derived growth factor
    • Low affinity platelet factor IV
    • Macrophage derived growth factor
    • MDGF
    • NAP 2
    • Neutrophil activating peptide 2
    • PBP
    • Platelet basic protein
    • Ppbp
    • pro platelet basic protein (chemokine (C X C motif) ligand 7)
    • SCYB7
    • Small inducible cytokine B7
    • Small inducible cytokine subfamily B, member 7
    • TC1
    • TC2
    • TCK 1
    • TGB
    • TGB1
    • THBGB
    • THBGB1
    • Thrombocidin 1
    • Thrombocidin 2
    • Thymus Chemokine 1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human CXCL7 ELISA Kit 图像

  • CXCL7 measured in biological fluids showing quantity (ng) per mL of tested sample

  • Representative Standard Curve using ab100613.

  • Representative Standard Curve using ab100613.

实验方案

Human CXCL7 ELISA Kit (ab100613)参考文献

ab100613 has not yet been referenced specifically in any publications.

Product Wall

The Assay Diluent B (1X) should be used to dilute the antibodies, regardless of what kind of samples are being assayed, plasma or other. Remember to dilute the diluent 5-fold (step 3): one part diluent stock and 4 parts deionized or distilled water.

Thank you for contacting us. The plates are from Greiner. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"