人CXCL11 ELISA试剂盒(I-TAC) (ab100580)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
- 产品名称人CXCL11 ELISA试剂盒(I-TAC)
参阅全部 CXCL11 试剂盒 - 检测方法Colorimetric
- 样品类型Cell culture supernatant, Serum, Plasma
- 检测类型Sandwich (quantitative)
- 灵敏度< 2 pg/ml
- 范围2.5 pg/ml - 600 pg/ml
- 复检
93 %
特定样本复检结果 样品类型 平均% 范围 Cell culture supernatant 92.82 80% - 105% Serum 95.28 84% - 106% Plasma 93.47 83% - 104% - 实验步骤Multiple steps standard assay
- 种属反应性与反应: Human
- 产品概述
Abcam’s Human CXCL11 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL11 in serum, plasma (heparin or EDTA), and cell culture supernatants.
This assay employs an antibody specific for Human CXCL11 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL11 C bound. The stop solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human CXCL11 ELISA Kit (I-TAC) (ab187392) from our SimpleStep ELISA® range.
- 说明
Optimization may be required with urine samples.
- 经测试应用适用于: Sandwich ELISAmore details
- 平台Microplate
性能
- 存放说明Store at -20°C. Please refer to protocols.
-
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CXCL11 2 vials CXCL11 Microplate (12 strips x 8 wells) 1 unit Recombinant Human CXCL11 Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml - 研究领域
- 功能Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
- 组织特异性High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
- 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
- 细胞定位Secreted.
- Information by UniProt
-
别名
- b R1
- Beta R1
- Beta-R1
see all -
数据库链接
- Entrez Gene: 6373 Human
- Omim: 604852 Human
- SwissProt: O14625 Human
- Unigene: 632592 Human
相关产品
- SimpleStep ELISA kits
应用
Our Abpromise guarantee covers the use of ab100580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Human CXCL11 ELISA Kit (I-TAC) 图像
-
Typical Standard CurveRepresentative Standard Curve using ab100580.
-
Typical Standard CurveRepresentative Standard Curve using ab100580.
实验方案
Human CXCL11 ELISA Kit (I-TAC) (ab100580)参考文献
This product has been referenced in:
- Asemota OO et al. Chemokines Responses to Ascaris Lumbricoides Sole Infection and Co-infection with Hookworm among Nigerians. N Am J Med Sci 6:84-8 (2014). Sandwich ELISA ; Human . Read more (PubMed: 24695523) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"