人CXCL11 ELISA试剂盒(I-TAC) (ab100580)

概述

  • 产品名称人CXCL11 ELISA试剂盒(I-TAC)
    参阅全部 CXCL11 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 2 pg/ml
  • 范围
    2.5 pg/ml - 600 pg/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 92.82 80% - 105%
    Serum 95.28 84% - 106%
    Plasma 93.47 83% - 104%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human CXCL11 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL11 in serum, plasma (heparin or EDTA), and cell culture supernatants.

    This assay employs an antibody specific for Human CXCL11 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL11 C bound. The stop solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
  • 组织特异性High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • b R1
    • Beta R1
    • Beta-R1
    • betaR1
    • C X C motif chemokine 11
    • C-X-C motif chemokine 11
    • Chemokine (C-X-C motif) ligand 11
    • Chemokine C-X-C motif ligand 11
    • CXCL11
    • CXL11_HUMAN
    • H174
    • I TAC
    • I-TAC
    • Interferon gamma inducible protein 9
    • Interferon gamma-inducible protein 9
    • Interferon inducible T cell a chemoattractant I TAC
    • Interferon inducible T cell alpha chemoattractant
    • Interferon-inducible T-cell alpha chemoattractant
    • IP 9
    • IP-9
    • IP9
    • ITAC
    • MGC102770
    • SCYB11
    • SCYB9B
    • Small inducible cytokine B11
    • Small inducible cytokine subfamily B (Cys X Cys) member 11
    • Small inducible cytokine subfamily B (Cys X Cys) member 9B
    • Small inducible cytokine subfamily B, member 11
    • Small inducible cytokine subfamily B, member 9B
    • Small-inducible cytokine B11
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human CXCL11 ELISA Kit (I-TAC) 图像

  • Representative Standard Curve using ab100580.

  • Representative Standard Curve using ab100580.

实验方案

Human CXCL11 ELISA Kit (I-TAC) (ab100580)参考文献

This product has been referenced in:
  • Asemota OO  et al. Chemokines Responses to Ascaris Lumbricoides Sole Infection and Co-infection with Hookworm among Nigerians. N Am J Med Sci 6:84-8 (2014). Sandwich ELISA ; Human . Read more (PubMed: 24695523) »

See 1 Publication for this product

Product Wall

I-TAC-CXCL11 human macrophage secretion

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I-TAC-CXCL11 secretion by human macrophages isolated by negative selection in culture for 3 & 7 days.
Kit easy to use, the assay takes about 6hr, good specificity as shown by higher secretion by M1 macrophages (MSCF+IFNg) on day 7 compared to the other time point and macrophage subtypes (M0=MCSF M2a=MCSF+IL4 DEX=MCSF+Dexamethasone IL10=MCSF+IL10 TGFb=MCSF+TGFb)
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提交于 Apr 09 2014

Veuillez trouver ci-après la réponse que j'ai obtenu du labo concernant le problème rencontré avec les kits ELISA ab100580 et ab100506. Laréponse est en anglais, j'espère que cela ne vousdérange pas. Tha...

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Merci de m'avoir envoyé le fichier Excel avec vos résultats. J'ai noté que seul l'axe absices (concentrations pg/ml) était logarythmique, mais pas l'axe des DO à 450nm. pourriez-vous s'il vous plait refaire et m'envo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"