人Cu/Zn Superoxide Dismutase ELISA试剂盒(ab119520)

概述

  • 产品名称人Cu/Zn Superoxide Dismutase ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 5.1%
    批次间
    样品 n Mean SD CV%
    Overall 5.8%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.04 ng/ml
  • 范围
    0.08 ng/ml - 5 ng/ml
  • 复检

    98 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 98 89% - 108%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Cu/Zn Superoxide Dismutase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cu/Zn Superoxide Dismutase concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate, heparin), amniotic fluid, fetal umbilical vein blood and urine.

    Cu/Zn Superoxide Dismutase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a Cu/Zn Superoxide Dismutase HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the Cu/Zn Superoxide Dismutase amount of sample captured in plate.

  • 说明

    ab119520 Cu/Zn Superoxide Dismutase Human ELISA kit has been shown to detect both SOD1 and SOD3.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • 疾病相关Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • 序列相似性Belongs to the Cu-Zn superoxide dismutase family.
  • 翻译后修饰Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • 细胞定位Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt
  • 别名
    • ALS
    • ALS1
    • Cu/Zn superoxide dismutase
    • homodimer
    • hSod1
    • Indophenoloxidase A
    • IPOA
    • SOD
    • SOD soluble
    • SOD1
    • SOD3
    • SODC_HUMAN
    • Superoxide dismutase [Cu-Zn]
    • Superoxide dismutase 1
    • Superoxide dismutase 1 soluble
    • Superoxide dismutase 3
    • Superoxide dismutase cystolic
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Cu/Zn Superoxide Dismutase ELISA Kit 图像

  • Representative Standard Curve using ab119520.

实验方案

Human Cu/Zn Superoxide Dismutase ELISA Kit (ab119520)参考文献

ab119520 has not yet been referenced specifically in any publications.

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Thank you for your call last week and for your patience while I have been in touch with the lab about your enquiry.

Theepitopse of the antibodies used in the kit have not been mapped at this point, and the kit has never been tested with sampl...

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Thank you for contacting us with your enquiry. I have looked through our catalogue and have found two sets that may be of interest to use. ab88404: http://www.abcam.com/Superoxide-Dismutase-1-antibody-ab88404.html and it's peptide ab99369: http...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"