概述

描述

  • 性质Synthetic
  • 氨基酸序列

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技术指标

Our Abpromise guarantee covers the use of ab38676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Blocking - Blocking peptide for Anti-CtIP antibody (ab38016)

  • 纯度70 - 90% by HPLC.

  • 形式Liquid
  • 补充说明

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

常规信息

  • 别名
    • COM1
    • COM1_HUMAN
    • CtBP interacting protein
    • CtBP-interacting protein
    • CtIP
    • DNA endonuclease RBBP8
    • JWDS
    • RBBP-8
    • RBBP8
    • Retinoblastoma-binding protein 8
    • Retinoblastoma-interacting protein and myosin-like
    • Rim
    • SAE2
    • SCKL2
    • Sporulation in the absence of SPO11 protein 2 homolog
    see all
  • 功能Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations.
  • 疾病相关Seckel syndrome 2
    Jawad syndrome
    Genetic variability in RBBP8 is noted as a factor in BRCA1-associated breast cancer risk (PubMed:21799032). Exhibits sensitivity to tamoxifen in certain breast cancer cell lines (PubMed:18171986).
  • 序列相似性Belongs to the COM1/SAE2/CtIP family.
  • 结构域The PXDLS motif binds to a cleft in CtBP proteins.
    The damage-recruitment motif is required for DNA binding and translocation to sites of DNA damage.
  • 翻译后修饰Acetylated. Deacetylation by SIRT6 upon DNA damage promotes DNA end resection.
    Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylation at Thr-847 by CDK1 is essential for the recruitment to DNA and the DNA repair function. Phosphorylated on Ser-327 as cells enter G2 phase. This phosphorylation is required for binding BRCA1 and for the G2/M DNA damage transition checkpoint control.
    Ubiquitinated (PubMed:14654780, PubMed:16818604). Ubiquitination at multiple sites by BRCA1 (via its N-terminal RING domain) does not lead to its proteosomal degradation but instead the ubiquitinated RBBP8 binds to chromatin following DNA damage and may play a role in G2/M checkpoint control (PubMed:16818604). Ubiquitinated by RNF138 at its N-terminus (PubMed:26502057).
  • 细胞定位Nucleus. Chromosome. Associates with sites of DNA damage in S/G2 phase (PubMed:10764811). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604).
  • Information by UniProt

Human CtIP peptide (ab38676)参考文献

ab38676 has not yet been referenced specifically in any publications.

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