概述

  • 产品名称人Complement C6 ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.1%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.7 ng/ml
  • 范围
    0.78 ng/ml - 50 ng/ml
  • 复检

    97 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Complement C6 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C6 in Human plasma, serum, saliva, urine, milk, and cell culture supernatants.

    A Complement C6 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C6 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C6 captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.
  • 疾病相关Defects in C6 are the cause of complement component 6 deficiency (C6D) [MIM:612446]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
  • 序列相似性Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 EGF-like domain.
    Contains 2 Kazal-like domains.
    Contains 1 LDL-receptor class A domain.
    Contains 1 MACPF domain.
    Contains 2 Sushi (CCP/SCR) domains.
    Contains 3 TSP type-1 domains.
  • 翻译后修饰All cysteine residues are assumed to be cross-linked to one another. Individual modules containing an even number of conserved cysteine residues are supposed to have disulfide linkages only within the same module.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • C6
    • CO6_HUMAN
    • Complement component C6
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab125965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Complement C6 ELISA Kit 图像

  • Complement C6 measured in various biofluids showing quantity (microgram) per mL of sample tested

  • Colorimetric standard curve: mean of duplicates (+/-) with background readings subtracted

实验方案

Human Complement C6 ELISA Kit (ab125965)参考文献

ab125965 has not yet been referenced specifically in any publications.

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