概述

  • 产品名称人Complement C5 ELISA试剂盒
    参阅全部 Complement C5 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 2.8%
    批次间
    样品 n Mean SD CV%
    Overall 12.6%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.1 ng/ml
  • 范围
    0.313 ng/ml - 20 ng/ml
  • 复检

    101 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Complement C5 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C5 in Human plasma, serum, saliva, milk, CSF and cell culture supernatants.

    A Complement C5 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C5 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C5 captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
    Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
  • 疾病相关Defects in C5 are the cause of complement component 5 deficiency (C5D) [MIM:609536]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
    Note=An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
  • 序列相似性Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • anaphylatoxin C5a analog
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
    • C5
    • CO5_HUMAN
    • complement C5
    • Complement C5 alpha'' chain
    • Complement component 5
    • CPAMD4
    • ECLZB
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab125963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Complement C5 ELISA Kit 图像

  • Complement 5 measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab125963

实验方案

Human Complement C5 ELISA Kit (ab125963)参考文献

This product has been referenced in:
  • Owsley C  et al. Associations between abnormal rod-mediated dark adaptation and health and functioning in older adults with normal macular health. Invest Ophthalmol Vis Sci 55:4776-89 (2014). ELISA ; Human . Read more (PubMed: 24854857) »

See 1 Publication for this product

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Thank you for contacting Abcam regarding ab125963.


I have confirmed that this kit will react withboth C5 and C5a.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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The kit standard is purified from human plasma with >95% purity by SDS-PAGE. The MW is 190,000 Da (2 chains).

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