人colon组织裂解液(adenocarcinoma) - insoluble fraction (female, 46 years) (ab44176)

概述

  • 产品名称人colon组织裂解液(adenocarcinoma) - insoluble fraction (female, 46 years)
    参阅全部 Colon tissue lysate 细胞裂解液
  • 描述
    人colon insoluble fraction裂解液
  • 常规说明

    Tissue: Colon Location: Rectum Diagnosis: Adenocarcinoma, well differentiated Stage: II T3NoMo Grade: I Gross findings: Tumor size 4 x 3 x 2 cm. Cut section is soft, white. Regional lymph nodes: 3. Microscopic findings: Tissue sections show proliferation of malignant epithelial glands. Epithelial cells have small basophilic, irregular nuclei and abundant cytoplasm. Cytoplasm contains muscin. Tumor cells arranged in glands. Mitotic figures are not evident. The tumor cells invaded the muscularis propria and subserosa. The stroma is infiltrated by large numbers of inflammatory cells. Tumor necrosis and blood vessel invasion are not revealed. Lymph nodes show hyperplasia.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44176: Extraction 1: soluble protein fraction ab44174 Human Colon Tumor Tissue lysate ab44173 Human Colon Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44176 Human Colon Tumor Tissue lysate ab44175 Human Colon Normal Tissue lysate (matched)

性能

  • 形式Liquid
  • 存放说明Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • 纯化说明Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    Human colon tissue lysate (adenocarcinoma) - insoluble fraction (female, 46 years) (ab44176)参考文献

    ab44176 has not yet been referenced specifically in any publications.

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