人Anti-Clostridium tetani toxin IgG ELISA试剂盒(ab108771)

概述

  • 产品名称
    人Anti-Clostridium tetani toxin IgG ELISA试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Weak + Serum 24 0.56U/ml 7.1%
    Pos. Serum 24 1.24U/ml 4.9%
    批次间
    样品 n Mean SD CV%
    Weak + Serum 10 0.22U/ml 11.3%
    Pos. Serum 10 0.54U/ml 7.7%
  • 样品类型
    Serum, Plasma
  • 检测类型
    Indirect
  • 灵敏度
    < 0.05 U/ml
  • 范围
    0.05 U/ml - 1 U/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s anti-Clostridium tetani toxin IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Clostridium tetani toxin in Human serum and plasma.

     

    A 96-well plate has been precoated with Clostridium tetani toxin antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Clostridium tetani toxin-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Clostridium tetani toxin IgG sample captured in plate.

  • 经测试应用
    适用于: Indirect ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Indirect ELISA Use at an assay dependent dilution.

图片

  • Representative Standard Curve using ab108771

实验方案

文献

ab108771 has not yet been referenced specifically in any publications.

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