人cervix组织裂解液(squamous cell cervical carcinoma) - insoluble fraction (female, 46 yrs) (ab43940)

概述

  • 产品名称人cervix组织裂解液(squamous cell cervical carcinoma) - insoluble fraction (female, 46 yrs)
    参阅全部 Cervix 细胞裂解液
  • 描述
    人cervix insoluble fraction
  • 常规说明

    Tissue: Cervix uteri Location: Cervix. Diagnosis: Squamous cell cervical carcinoma, well differentiated. Stage: IA1 T1A1N0M0 Grade: 1.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insouble protein fraction. Please find below a range of products that relate to ab43940: Extraction 1: soluble protein fraction ab43937 Human cervix tumor tissue lysate ab43938 Human cervix normal tissue lysate (matched) Extraction 2: insoluble protein fraction ab43940 Human cervix tumor tissue lysate ab43939 Human cervix normal tissue lysate (matched)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.40
    Constituents: PBS, 0.77% DTT, 0.1% SDS, 30.03% Urea, 15.22% Thiourea, 100% Laemmli buffer
    Note: 20µl of 5X Laemmli buffer were added per 100µl of tissue lysate
  • Concentration information loading...
  • 纯化说明Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • 研究领域

    Human cervix tissue lysate (squamous cell cervical carcinoma) - insoluble fraction (female, 46 yrs) (ab43940)参考文献

    ab43940 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"