人CD95 ELISA试剂盒(TNFRSF6) (ab45922)

概述

  • 产品名称
    人CD95 ELISA试剂盒(TNFRSF6)
    参阅全部 Fas 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 14 1719pg/ml 106 6.1%
    Sample B 16 703pg/ml 39 5.5%
    批次间
    样品 n Mean SD CV%
    Sample A 8 1752pg/ml 145 8.2%
    Sample B 8 856pg/ml 68 7.9%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 47 pg/ml
  • 范围
    93.75 pg/ml - 3000 pg/ml
  • 检测时间
    1h 45m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human soluble CD95 (TNFRSF6) ELISA is to be used for the in-vitro quantitative determination of soluble CD95 in Human serum, plasma, buffered solutions or cell culture medium.


    A monoclonal antibody specific for CD95 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD95 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD95 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD95 present in the samples. 


    This kit will recognize both endogenous and recombinant Human CD95.


    Get results in 90 minutes with Human FASLG Receptor ELISA Kit (CD95) (ab183360) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-CD95 Red 1 x 0.4ml 2 x 0.4ml
    CD95 Control Silver 2 vials 4 vials
    CD95 Microplate (12 x 8 well strips) 1 unit 2 units
    CD95 Standard (Lyophilized) Yellow 2 vials 4 vials
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究领域
  • 功能
    Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
  • 组织特异性
    Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
  • 疾病相关
    Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • 序列相似性
    Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • 结构域
    Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
  • 细胞定位
    Secreted and Cell membrane.
  • Information by UniProt
  • 别名
    • ALPS 1A
    • ALPS1A
    • APO 1
    • Apo 1 antigen
    • APO 1 cell surface antigen
    • Apo-1 antigen
    • APO1
    • Apo1 antigen
    • APO1 cell surface antigen
    • Apoptosis antigen 1
    • Apoptosis mediating surface antigen FAS
    • Apoptosis-mediating surface antigen FAS
    • APT 1
    • APT1
    • CD 95
    • CD 95 antigen
    • CD95
    • CD95 antigen
    • Delta Fas
    • Delta Fas/APO 1/CD95
    • Delta Fas/APO1/CD95
    • Fas
    • Fas (TNF receptor superfamily, member 6)
    • FAS 1
    • FAS 827dupA
    • Fas AMA
    • FAS Antigen
    • Fas cell surface death receptor
    • FAS1
    • FASLG receptor
    • FASTM
    • sFAS
    • Surface antigen APO1
    • TNF receptor superfamily, member 6
    • TNFRSF 6
    • TNFRSF6
    • TNR6_HUMAN
    • Tumor necrosis factor receptor superfamily member 6
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab45922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab45922

实验方案

文献

ab45922 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your enquiry.

I have reviewed the details on the datasheets for these kits. For the Fas ligand kits:

ab45892 Fas Ligand Human ELISA Kit
ab100515 Fas Ligand Human ELISA Kit

Sensitivities are different:...

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Thank you for your inquiry.

These ELISA kits are from two different sources but they are similar.

I recommend todetermine which is the best foryour needs from the datasheet and protocol information. Please take not of the different ...

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Thank for your quick response. As I have noted in my previous e-mail, the kit seems to be working since the standard curve obtained is fine. The actual standard values are concentration-dependent and the curve is linear. We assayed over 50 health...

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Thank for your enquiry regarding ab45922 and for providing some data saved in Excel file. After reviewing the results you have kindly sent to us, the standard curve obtained is fair and it seems the kit worked fine. Could you please confirm t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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