人CD95 ELISA试剂盒(TNFRSF6) (ab45922)

概述

  • 产品名称人CD95 ELISA试剂盒(TNFRSF6)
    参阅全部 CD95 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 14 1719pg/ml 106 6.1%
    Sample B 16 703pg/ml 39 5.5%
    批次间
    样品 n Mean SD CV%
    Sample A 8 1752pg/ml 145 8.2%
    Sample B 8 856pg/ml 68 7.9%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 47 pg/ml
  • 范围
    93.75 pg/ml - 3000 pg/ml
  • 检测时间
    1h 45m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human soluble CD95 (TNFRSF6) ELISA is to be used for the in-vitro quantitative determination of soluble CD95 in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for CD95 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD95 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD95 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD95 present in the samples. 

    This kit will recognize both endogenous and recombinant Human CD95.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
  • 组织特异性Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
  • 疾病相关Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • 序列相似性Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • 结构域Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 别名
    • ALPS1A
    • APO 1
    • APO 1 cell surface antigen
    • Apo-1 antigen
    • APO1
    • Apo1 antigen
    • Apoptosis antigen 1
    • Apoptosis mediating surface antigen FAS
    • Apoptosis-mediating surface antigen FAS
    • APT1
    • CD95
    • CD95 antigen
    • Fas
    • Fas AMA
    • Fas antigen
    • FAS1
    • FASLG receptor
    • FASTM
    • TNF receptor superfamily member 6
    • TNFRSF6
    • TNR6_HUMAN
    • Tumor necrosis factor receptor superfamily member 6
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab45922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human CD95 ELISA Kit (TNFRSF6) 图像

  • Representative Standard Curve using ab45922

实验方案

Human CD95 ELISA Kit (TNFRSF6) (ab45922)参考文献

ab45922 has not yet been referenced specifically in any publications.

Product Wall

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