概述

  • 产品名称人CD62L ELISA试剂盒(Selectin L)
    参阅全部 CD62L 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 15 28ng/ml 1.3 4.6%
    Sample B 19 9ng/ml 0.5 5.5%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 1 ng/ml
  • 范围
    1.6 ng/ml - 60 ng/ml
  • 检测时间
    1h 45m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CD62L (Selectin L) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD62L in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for CD62L has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD62L concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD62L are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD62L present in the samples. 

    This kit will recognize both endogenous and recombinant Human CD62L.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性CD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.
  • 细胞定位Cell Membrane; single-pass type I membrane protein.
  • 别名
    • A.11
    • AI528707
    • CD62 antigen ligand
    • CD62 antigen-like family member L
    • gp90 MEL
    • hLHRc
    • IgA nephropathy, susceptibility to, included
    • L Selectin
    • LAM1
    • LECAM1
    • Leu8
    • Leukocyte adhesion molecule 1
    • Leukocyte surface antigen Leu8
    • Leukocyte-endothelial cell adhesion molecule 1
    • LNHR
    • LSEL
    • Ly-22
    • Ly-m22
    • LYAM1
    • Lymph node homing receptor
    • Lymphocyte adhesion molecule 1
    • Lymphocyte antigen 22
    • Lymphocyte surface MEL-14 antigen
    • PLNHR
    • Selectin L
    • Selectin, lymphocyte
    • SELL
    • TQ1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab45917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human CD62L ELISA Kit (Selectin L) 图像

  • Representative Standard Curve using ab45917

实验方案

Human CD62L ELISA Kit (Selectin L) (ab45917)参考文献

This product has been referenced in:
  • Chen R  et al. Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions. PLoS Comput Biol 6: (2010). ELISA ; Human . Read more (PubMed: 20885780) »

See 1 Publication for this product

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