人CD62E ELISA试剂盒(Selectin) (ab45914)

概述

  • 产品名称
    人CD62E ELISA试剂盒(Selectin)
    参阅全部 CD62E 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 12 31.55ng/ml 0.272 0.86%
    B 12 4.22ng/ml 0.174 4.11%
    批次间
    样品 n Mean SD CV%
    A 32 31.34ng/ml 0.477 1.52%
    B 32 4.18ng/ml 0.266 6.36%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 0.5 ng/ml
  • 范围
    1 ng/ml - 32 ng/ml
  • 检测时间
    2h 10m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CD62E (Selectin) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble CD62E in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for CD62E has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD62E concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD62E are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD62E present in the samples. 

    This kit will recognize both endogenous and recombinant Human CD62E.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab45914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • CD62E measured in RIPA-lysates of  control (CTRL) or in HUVEC cells stimulated with LPS (Sigma) for 6 hours (0.5 microgram x mL-1 LPS) or for 24 hours (0.5 microgram x mL-1 LPS for the first 18 hours, 1 microgram x mL-1 for the last 6 hours). Results shown as ng per million cells (duplicates +/- SD). Samples tested at 0.5-20 million lysed cells per mL.

  • CD62E measured in biological fluids (dilution range 1/1-1/20; duplicates +/- SD).

  • Representative Standard Curve using ab45914

实验方案

文献

ab45914 has not yet been referenced specifically in any publications.

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